Pre-and Postoperative Circulating Tumour DNA in Patients With Gastrointestinal Stromal Tumour – A Methodological Assessment Study
| العنوان: | Pre-and Postoperative Circulating Tumour DNA in Patients With Gastrointestinal Stromal Tumour – A Methodological Assessment Study |
|---|---|
| المؤلفون: | CHARLOTTE MARGARETA BRINCH, NINNA AGGERHOLM-PEDERSEN, TIM SVENSTRUP POULSEN, WOJCIECH SKOVRIDER-RUMINSKI, PIETER DE HEER, LUIT PENNINGA, ANDERS KRARUP-HANSEN, ESTRID HOGDALL |
| المصدر: | Brinch, C M, Aggerholm-Pedersen, N, Poulsen, T S, Skovrider-Ruminski, W, DE Heer, P, Penninga, L, Krarup-Hansen, A & Hogdall, E 2022, ' Pre-and Postoperative Circulating Tumour DNA in Patients With Gastrointestinal Stromal Tumour-A Methodological Assessment Study ', Anticancer Research, vol. 42, no. 11, pp. 5527-5537 . https://doi.org/10.21873/anticanres.16022Test |
| بيانات النشر: | Anticancer Research USA Inc., 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cancer Research, Receptor, Platelet-Derived Growth Factor alpha, Gastrointestinal Stromal Tumors, Gastrointestinal stromal tumour, circulating tumour DNA, General Medicine, Protein-Tyrosine Kinases, Circulating Tumor DNA, Proto-Oncogene Proteins c-kit, Oncology, Mutation, Humans, Receptors, Platelet-Derived Growth Factor, next-generation sequencing, Cell-Free Nucleic Acids |
| الوصف: | BACKGROUND/AIM: Gastrointestinal stromal tumours (GISTs) harbour genetic aberrations in receptor tyrosine kinase KIT (KIT) or platelet-derived growth factor receptor A (PDGFRA) in 85-90% of the patients. Circulating tumour DNA (ctDNA) is a potential biomarker in patients with GIST. Previous studies investigating ctDNA around surgery in patients with GIST presented divergent results regarding the proportion of patients with detectable ctDNA. This study aimed to 1) investigate the feasibility of detecting and monitoring ctDNA pre-and postoperative, 2) compare two different circulating free DNA (cfDNA) extraction methods, and validate results obtained by next-generation sequencing (NGS) using Real-Time PCR technology. PATIENTS AND METHODS: Eight patients planned for immediate surgery or surgery after neoadjuvant oncological treatment were included in the study, from whom blood collection was performed pre- and postoperatively for ctDNA analysis. Furthermore, blood samples from six patients with GIST harbouring a point mutation in KIT or PDGFRA in tissues from primary tumours were used for comparison and validation sub-study. RESULTS: In this explorative study, none of the patients with very low to intermediate risk GIST harboured KIT, or PDGFRA mutated ctDNA in pre-or postoperative blood samples. The methods used for cfDNA extraction gave similar output, and the two methods for ctDNA analysis gave identical results. CONCLUSION: There is no benefit in analysing ctDNA around surgery in very low to intermediate-risk GIST patients. Larger studies investigating ctDNA in patients with high-risk GIST around surgery are warranted. |
| تدمد: | 1791-7530 0250-7005 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::135ec9b3db6a677d95e3e19bb063bf19Test https://doi.org/10.21873/anticanres.16022Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....135ec9b3db6a677d95e3e19bb063bf19 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917530 02507005 |
|---|