Perampanel Inhibits Neuroblastoma Cell Proliferation Through Down-regulation of AKT and ERK Pathways
| العنوان: | Perampanel Inhibits Neuroblastoma Cell Proliferation Through Down-regulation of AKT and ERK Pathways |
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| المؤلفون: | Michio Ozeki, Shiho Yasue, Toshiyuki Fukao, Norio Kawamoto, Hidenori Ohnishi, Akifumi Nozawa, Mina Nakama, Saori Endo, Mikako Matsuoka |
| المصدر: | Anticancer Research. 39:3595-3599 |
| بيانات النشر: | Anticancer Research USA Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | MAPK/ERK pathway, Cancer Research, Pyridones, Down-Regulation, Antineoplastic Agents, Neuroblastoma, 03 medical and health sciences, Perampanel, chemistry.chemical_compound, 0302 clinical medicine, Cell Line, Tumor, Nitriles, medicine, Humans, Receptors, AMPA, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Protein kinase B, PI3K/AKT/mTOR pathway, Cell Proliferation, Cell growth, Kinase, General Medicine, medicine.disease, Oncology, chemistry, 030220 oncology & carcinogenesis, Cancer research, Proto-Oncogene Proteins c-akt |
| الوصف: | BACKGROUND/AIM Activation of AKT serine/ threonine kinase (AKT) predicts poor outcome in neuroblastoma, which highlights the potential of the AKT pathway as a promising target for neuroblastoma treatment. Several studies reported that blockade of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptors (AMPARs) reduces proliferation in glioblastoma or lung cancer by inhibiting AKT and extracellular signal-related kinase (ERK) pathways. In this study, we examined the effect of the AMPAR antagonist perampanel on human neuroblastoma cells. MATERIALS AND METHODS Cell proliferation, caspase activity, and western blot assays were performed to determine the effect of perampanel on the KP-N-SI9s human neuroblastoma cell line. RESULTS Perampanel inhibited cell proliferation without triggering apoptosis in neuroblastoma cells. Down-regulation of AKT protein levels, AKT phosphorylation, and ERK1/2 phosphorylation were also observed in neuroblastoma cells with perampanel treatment. CONCLUSION Perampanel inhibits neuroblastoma cell proliferation through down-regulation of AKT and ERK pathways and has potential for the treatment of neuroblastoma. |
| تدمد: | 1791-7530 0250-7005 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c056ad531f32fff00105c0e822c2822Test https://doi.org/10.21873/anticanres.13506Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1c056ad531f32fff00105c0e822c2822 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917530 02507005 |
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