Identification of Functional Thyroid Stimulating Hormone Receptor and TSHR Gene Mutations in Hepatocellular Carcinoma
| العنوان: | Identification of Functional Thyroid Stimulating Hormone Receptor and TSHR Gene Mutations in Hepatocellular Carcinoma |
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| المؤلفون: | Yu-De Chu, Ya-Hui Huang, Chau-Ting Yeh, Kwang-Huei Lin, Yu-Lin Shih |
| المصدر: | Anticancer Research. 38 |
| بيانات النشر: | Anticancer Research USA Inc., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, endocrine system, Cancer Research, endocrine system diseases, Cell, Biology, Gene mutation, 03 medical and health sciences, chemistry.chemical_compound, Thyroid-stimulating hormone, medicine, Cyclic adenosine monophosphate, Receptor, General Medicine, HCCS, medicine.disease, digestive system diseases, eye diseases, 030104 developmental biology, medicine.anatomical_structure, Oncology, chemistry, Hepatocellular carcinoma, Cancer research, Immunohistochemistry, hormones, hormone substitutes, and hormone antagonists |
| الوصف: | Background/aim Extra-thyroid expression of thyroid stimulating hormone (TSH) receptor (TSHR) has been reported in normal liver tissues, but never assessed in hepatocellular carcinoma (HCC). Patients and methods Paired cancerous and non-cancerous HCC tissues were analyzed with TSHR expression assays. TSHR functional assessments and sequence analysis for the TSHR exon-10 were performed. Results TSHR overexpression was found in 150/197 (76.1%) HCCs. Higher TSHR expression was associated with unfavorable postoperative outcomes. Immunohistochemical analysis revealed predominantly nuclei/peri-nuclei localization of TSHR in cancerous tissues but cell membrane localization in non-cancerous parts. TSH stimulation on hepatoma cells resulted in increased cyclic adenosine monophosphate levels with altered cell sensitivity to cisplatin. Gene mutations leading to TSHR truncation were detected in 8/81 (9.9%) HCC tissues. Conclusion Overexpression of TSHR was found in a great majority of HCC tissues and associated with unfavorable prognosis. Cell-based experiments and gene mutation analysis suggested that TSHR in HCCs was functional. |
| تدمد: | 1791-7530 0250-7005 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ad9b0bb128e8e3ab82226057c371489bTest https://doi.org/10.21873/anticanres.12523Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........ad9b0bb128e8e3ab82226057c371489b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917530 02507005 |
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