دورية أكاديمية
Ultrasensitive Measurement of Ca(2+) Influx into Lipid Vesicles Induced by Protein Aggregates.
| العنوان: | Ultrasensitive Measurement of Ca(2+) Influx into Lipid Vesicles Induced by Protein Aggregates. |
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| المؤلفون: | Flagmeier, P, De, S, Wirthensohn, DC, Lee, SF, Vincke, C, Muyldermans, S, Knowles, TPJ, Gandhi, S, Dobson, CM, Klenerman, D |
| بيانات النشر: | John Wiley & Sons Ltd. //dx.doi.org/10.1002/anie.201700966 Angewandte Chemie - International Edition |
| سنة النشر: | 2017 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Alzheimer's disease, fluorescence imaging, nano-scale biophysics, neurodegeneration, protein aggregation |
| الوصف: | To quantify and characterize the potentially toxic protein aggregates associated with neurodegenerative diseases, a high-throughput assay based on measuring the extent of aggregate-induced Ca(2+) entry into individual lipid vesicles has been developed. This approach was implemented by tethering vesicles containing a Ca(2+) sensitive fluorescent dye to a passivated surface and measuring changes in the fluorescence as a result of membrane disruption using total internal reflection microscopy. Picomolar concentrations of Aβ42 oligomers could be observed to induce Ca(2+) influx, which could be inhibited by the addition of a naturally occurring chaperone and a nanobody designed to bind to the Aβ peptide. We show that the assay can be used to study aggregates from other proteins, such as α-synuclein, and to probe the effects of complex biofluids, such as cerebrospinal fluid, and thus has wide applicability. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/265901Test |
| DOI: | 10.17863/CAM.12271 |
| الإتاحة: | https://doi.org/10.17863/CAM.12271Test https://www.repository.cam.ac.uk/handle/1810/265901Test |
| رقم الانضمام: | edsbas.D02D6447 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.12271 |
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