دورية أكاديمية
Urine metabolomics reveals new insights into hyperlipidemia and the therapeutic effect of rhubarb
| العنوان: | Urine metabolomics reveals new insights into hyperlipidemia and the therapeutic effect of rhubarb |
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| المؤلفون: | Chen, Hua, Yuan, Bin, Miao, Hua, Tan, Yi, Bai, Xu, Zhao, Ying-Yong, Wang, Yun |
| المساهمون: | Zhao, YY (reprint author), Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, 229 Taibai North Rd, Xian 710069, Shaanxi, Peoples R China., Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, Xian 710069, Shaanxi, Peoples R China., Univ Calif Irvine, Sch Med, Div Nephrol & Hypertens, MedSci 1, Irvine, CA 92897 USA., Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China., Waters Technol Shanghai Ltd, Solut Ctr, Shanghai 201203, Peoples R China., Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100029, Peoples R China., Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, 229 Taibai North Rd, Xian 710069, Shaanxi, Peoples R China. |
| المصدر: | SCI ; EI |
| بيانات النشر: | analytical methods |
| سنة النشر: | 2015 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | CHRONIC-RENAL-FAILURE, DIET-INDUCED HYPERLIPIDEMIA, CHRONIC KIDNEY-DISEASE, UPLC-Q-TOF/HSMS/MSE, MASS-SPECTROMETRY, GRAMMAR SYSTEMS, PRISTANIC ACID, Q-TOF/MS, RATS, METABONOMICS |
| الوصف: | Rhubarb has been widely used for the treatment of hyperlipidemia and blood stasis. We conducted an ultra performance liquid chromatography-quadrupole time-of-flight high-definition mass spectrometry-based urinary metabolomic study on the diet-induced hyperlipidemic rats to present new insights into hyperlipidemia and the therapeutic effect of rhubarb. Coupled with blood biochemistry results, a significant difference in metabolic profiles among control, diet-induced hyperlipidemic and rhubarb-treated hyperlipidemic groups by partial least squares-discriminate analysis demonstrated that alterations in global urinary metabolites occurred significantly. Twenty-nine metabolites were identified as potential biomarkers by the accurate mass, isotopic pattern and MSE fragments information in both positive and negative ion modes. Significantly higher levels of octadecanamide, 3-methyluridine, oleamide, ascorbalamic acid, 1-methylnicotinamide, phenylacetylglycine, xanthine, aspartic acid, ribothymidine, 4-aminohippuric acid, methylhippuric acid, arginine and hippuric acid as well as significantly lower levels of 3-O-methyldopa, trimethyltridecanoic acid, S-cysteinosuccinic acid, indole-3-carboxylic acid, creatinine, carnitine, phenylalanine, 2-pyrroloylglycine, phytosphingosine, cyclohexanecarboxylic acid, propionylcarnitine, N-acetylneuraminic acid, cytidine, phenylpropionylglycine, palmitic acid and ribonic acid were observed in the diet-induced hyperlipidemic rats compared with the control rats. However, tendencies of these metabolites were obviously reversed in the rhubarb-treated hyperlipidemic rats. Changes of these metabolites were mainly associated with the perturbations of fatty acid metabolism, amino acid metabolism and nucleoside metabolism. The results indicated the amelioration and the therapeutic effect of rhubarb for hyperlipidemia, and further provided new insights into hyperlipidemia and the therapeutic effect of rhubarb. ; Chemistry, Analytical ; Food Science & Technology ; Spectroscopy ; SCI(E) ; EI ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1759-9660 1759-9679 |
| العلاقة: | ANALYTICAL METHODS.2015,7,(7),3113-3123.; 751314; http://hdl.handle.net/20.500.11897/206450Test; WOS:000351931000028 |
| DOI: | 10.1039/c5ay00023h |
| الإتاحة: | https://doi.org/20.500.11897/206450Test https://doi.org/10.1039/c5ay00023hTest https://hdl.handle.net/20.500.11897/206450Test |
| رقم الانضمام: | edsbas.653DA0B3 |
| قاعدة البيانات: | BASE |
| تدمد: | 17599660 17599679 |
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| DOI: | 10.1039/c5ay00023h |