المؤلفون: Nigel Lambert, HJ Carr, Terence J McMaster, Peter R. Shewry, J. M. Field, P. Cairns, Peter S. Belton, Mary A. Whittam, Arthur S. Tatham, D Jeenes, B Hanley, V.J. Morris, Mervyn J Miles

المصدر: Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films. 8:698-702

مصطلحات موضوعية: chemistry.chemical_classification, Chemistry, Biomolecule, Surfaces and Interfaces, Condensed Matter Physics, Surfaces, Coatings and Films, law.invention, chemistry.chemical_compound, Crystallography, Adsorption, Protein structure, law, Molecule, Graphite, Scanning tunneling microscope, Ethidium bromide, DNA

الوصف: We have used STM to study a range of biological molecules. The necessity of immobilizing the molecules on the surface was recognized and achieved by the formation of ordered layers or by the addition of chemical groups which strongly adsorbed to the graphite substrate. Clear images of the helical structure of the synthetic polypeptide poly γ‐benzyl‐L‐glutamate were obtained. Images of protein molecules isolated from wheat were found to be consistent with the rare β‐spiral conformation. The binding of the mutagen ethidium bromide to DNA was also studied and images of the cyclic glucan β‐cyclodextrin, tosylated to improve adsorption of the molecule to graphite, were also obtained. Although the contrast mechanism is unclear in some of these examples, these results suggest that STM can be applied successfully to a wide range of biomolecules.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::08e0d1b960fc72fadd209270ff142300Test
https://doi.org/10.1116/1.576986Test

المؤلفون: S.G. Ring, Arthur S. Tatham, Peter R. Shewry, Mervyn J Miles, Neil H. Thomson

المصدر: Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures. 12:1565

مصطلحات موضوعية: Microscope, Aqueous solution, biology, Starch, Atomic force microscopy, Granule (cell biology), General Engineering, food and beverages, Enzyme assay, law.invention, Crystallography, chemistry.chemical_compound, chemistry, Chemical engineering, law, biology.protein, Starch granule, Electron microscope

الوصف: Atomic force microscopy has been used to image wheat seed starch granules (cultivar Timmo) both in air and under water. In the aqueous environment, the enzyme α‐amylase was introduced into the solution cell of the microscope and its progressive attack on a starch granule was followed by collecting successive images of the granule. Each image was recorded over a time period of 105 s. The enzyme created a ‘‘pin‐hole’’ in the top of the granule, consistent with previous off‐line electron microscopy studies.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::4599d56209a430742305bcd4b866a15fTest
https://doi.org/10.1116/1.587287Test