Molecular Surveillance of Plasmodium falciparum Drug Resistance Markers in Clinical Samples from Botswana
| العنوان: | Molecular Surveillance of Plasmodium falciparum Drug Resistance Markers in Clinical Samples from Botswana |
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| المؤلفون: | Thato Motshoge, Moses Vurayai, Michela Menegon, Giacomo M. Paganotti, Tjantilili Mosweunyane, Ishmael Kasvosve, Leabaneng Tawe, Mariangela L’Episcopia, Wame Bothudile, Charles Muthoga, Carlo Severini, Naledi Mutukwa, Pleasure Ramatlho |
| المصدر: | The American Journal of Tropical Medicine and Hygiene. 99:1499-1503 |
| بيانات النشر: | American Society of Tropical Medicine and Hygiene, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Drug Resistance, Gene Dosage, Protozoan Proteins, Gene Expression, Drug resistance, chemistry.chemical_compound, Multiplicity of infection, Molecular marker, Malaria, Falciparum, Artemisinin, Child, Merozoite Surface Protein 1, Aged, 80 and over, Molecular Epidemiology, Botswana, biology, Articles, Middle Aged, Artemisinins, Dried blood spot, Infectious Diseases, Child, Preschool, Epidemiological Monitoring, Female, Multidrug Resistance-Associated Proteins, medicine.drug, Adult, Genetic Markers, Adolescent, Plasmodium falciparum, Calcium-Transporting ATPases, Lumefantrine, Antimalarials, 03 medical and health sciences, Virology, parasitic diseases, medicine, Animals, Humans, Genotyping, Aged, Polymorphism, Genetic, Infant, Membrane Transport Proteins, biology.organism_classification, 030104 developmental biology, Haplotypes, chemistry, Parasitology |
| الوصف: | Drug-resistant Plasmodium falciparum is a major threat to global malaria control and elimination efforts. In Botswana, a southern African country approaching malaria elimination, P. falciparum molecular data are not available. Parasites were assessed through pollymerase chain reaction (PCR) for confirmation of positive rapid diagnostic tests, multiplicity of infection (MOI), and drug resistance markers among isolates from clinical uncomplicated malaria cases collected at health facilities. Of 211 dried blood spot samples selected for the study, 186 (88.2%) were PCR positive for P. falciparum. The mean MOI based on MSP1 genotyping was 2.3 and was not associated with age. A high prevalence of wild-type parasites for pfcrt and pfmdr1 was found, with a haplotype frequency (K76/N86) of 88.8% and 17.7% of the isolates having two copies of the pfmdr1 gene. For pfATPase6, all the parasites carried the wild-type S769 allele. Sequencing showed no evidence of non-synonymous mutations associated with reduced artemisinin derivative sensitivity in the P. falciparum k13 gene. In conclusion, we found that P. falciparum parasites in Botswana were mostly wild type for the drug resistance markers evaluated. Yet, there was a high rate of a molecular marker associated to reduced sensitivity to lumefantrine. Our results indicate the need for systematic drug efficacy surveillance to complement malaria elimination efforts. |
| تدمد: | 1476-1645 0002-9637 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1bdf3eeef8ec360a2808ff192d7f30aTest https://doi.org/10.4269/ajtmh.18-0440Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f1bdf3eeef8ec360a2808ff192d7f30a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14761645 00029637 |
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