PYR/RCAR receptors contribute to Ozone-, Reduced Air Humidity-, Darkness- and CO2-Induced Stomatal Regulation
| العنوان: | PYR/RCAR receptors contribute to Ozone-, Reduced Air Humidity-, Darkness- and CO2-Induced Stomatal Regulation |
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| المؤلفون: | Ebe Merilo, Hannes Kollist, Ingmar Tulva, Miguel González-Guzmán, Mikael Brosché, Honghong Hu, Kristiina Laanemets, Pedro L. Rodriguez, Julian I. Schroeder, Liina Jakobson, Shaowu Xue |
| المصدر: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| بيانات النشر: | American Society of Plant Biologists, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Physiology, 2C protein phosphatases, Arabidopsis, Arabidopsis guard-cells, Plant Science, ABI2-1 mutants, chemistry.chemical_compound, Phosphoprotein Phosphatases, Arabidopsis thaliana, Receptor, Abscisic acid, Pyrabactin, biology, S-type anion, Intracellular Signaling Peptides and Proteins, food and beverages, Abscisic-acid, Channel SLAC1, Darkness, Protein Phosphatase 2C, Biochemistry, Signal transduction, Signal Transduction, Stomatal conductance, Hydrogen-Peroxide, Ozone, Genetics, BIOQUIMICA Y BIOLOGIA MOLECULAR, Relative-humidity, Ecophysiology and Sustainability, Arabidopsis Proteins, fungi, Membrane Proteins, Membrane Transport Proteins, Humidity, MICROBIOLOGIA, Carbon Dioxide, biology.organism_classification, chemistry, Signal-transduction, Mutation, Plant Stomata, Biophysics, Carrier Proteins, Protein Kinases, Plant-cells, Abscisic Acid |
| الوصف: | [EN] Rapid stomatal closure induced by changes in the environment, such as elevation of CO2, reduction of air humidity, darkness, and pulses of the air pollutant ozone (O-3), involves the SLOW ANION CHANNEL1 (SLAC1). SLAC1 is activated by OPEN STOMATA1 (OST1) and Ca2+-dependent protein kinases. OST1 activation is controlled through abscisic acid (ABA)-induced inhibition of type 2 protein phosphatases (PP2C) by PYRABACTIN RESISTANCE/REGULATORY COMPONENTS OF ABA RECEPTOR (PYR/RCAR) receptor proteins. To address the role of signaling through PYR/RCARs for whole-plant steady-state stomatal conductance and stomatal closure induced by environmental factors, we used a set of Arabidopsis (Arabidopsis thaliana) mutants defective in ABA metabolism/signaling. The stomatal conductance values varied severalfold among the studied mutants, indicating that basal ABA signaling through PYR/RCAR receptors plays a fundamental role in controlling whole-plant water loss through stomata. PYR/RCAR-dependent inhibition of PP2Cs was clearly required for rapid stomatal regulation in response to darkness, reduced air humidity, and O-3. Furthermore, PYR/RCAR proteins seem to function in a dose-dependent manner, and there is a functional diversity among them. Although a rapid stomatal response to elevated CO2 was evident in all but slac1 and ost1 mutants, the bicarbonate-induced activation of S-type anion channels was reduced in the dominant active PP2C mutants abi1-1 and abi2-1. Further experiments with a wider range of CO2 concentrations and analyses of stomatal response kinetics suggested that the ABA signalosome partially affects the CO2-induced stomatal response. Thus, we show that PYR/RCAR receptors play an important role for the whole-plant stomatal adjustments and responses to low humidity, darkness, and O-3 and are involved in responses to elevated CO2. This work was supported by the Estonian Ministry of Science and Education (grant no. IUT2-21), by the European Regional (Center of Excellence in Environmental Adaptation) and Social (Mobilitas Top Researchers grant no. MTT9) Fund, by the National Science Foundation (grant no. MCB0918220 to J.I.S.) and the National Institutes of Health (grant no. R01GM060396 to J.I.S.), by Shanxi Scholarship Council of China (grant no. 2011-012 to S.X.), by Shanxi Technology Foundation and Natural Science Foundation of Shanxi (grant no. 2012011006-4 to S.X.), and by Ministerio de Economia y Competitividad (grant no. BIO2011-23446 to P.L.R.). |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1104/pp.113.220608 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9cc0d90caf4d0a3e0184a05520ee492Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b9cc0d90caf4d0a3e0184a05520ee492 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1104/pp.113.220608 |
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