Reduced Ex Vivo Interleukin-8 Production by Neutrophils in Septic and Nonseptic Systemic Inflammatory Response Syndrome
| العنوان: | Reduced Ex Vivo Interleukin-8 Production by Neutrophils in Septic and Nonseptic Systemic Inflammatory Response Syndrome |
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| المؤلفون: | Didier Payen, Jean-Marc Cavaillon, Catherine Fitting, Christelle Marie, Jane Muret, Marie-Reine Losser |
| المصدر: | Blood. 91:3439-3446 |
| بيانات النشر: | American Society of Hematology, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Adult, Chemokine, Lipopolysaccharide, Neutrophils, medicine.medical_treatment, Immunology, Inflammation, Biology, Biochemistry, Sepsis, chemistry.chemical_compound, Escherichia coli, medicine, Humans, Aged, Interleukin-8, Cell Biology, Hematology, Middle Aged, medicine.disease, Systemic Inflammatory Response Syndrome, Interleukin-10, Endotoxins, Systemic inflammatory response syndrome, Interleukin 10, Cytokine, chemistry, General Surgery, biology.protein, Female, medicine.symptom, Ex vivo |
| الوصف: | Ex vivo cytokine production by circulating lymphocytes and monocytes is reduced in patients with infectious or noninfectious systemic inflammatory response syndrome. Very few studies have addressed the reactivity of polymorphonuclear cells (PMN). To analyze further the relative contribution of systemic inflammatory response syndrome alone or in combination with infection we studied the interleukin-8 (IL-8) production by PMN isolated from patients who had undergone cardiac surgery with cardiopulmonary bypass (CPB) and patients with sepsis. Cells were activated with either lipopolysaccharide (LPS) or heat-killed streptococci. Compared with healthy controls, the release of IL-8 by PMN in both groups of patients was significantly reduced whether activated by LPS, independently of its concentration and origin, or by heat-killed streptococci. These observations suggest that stressful conditions related to inflammation, independently of infection, rapidly dampened the reactivity of circulating PMN. We investigated whether the observed diminished reactivity of PMN might reflect an endotoxin tolerance phenomenon. Our in vitro experiments with PMN from healthy controls indicated that PMN could not be rendered tolerant stricto sensu. However, our data suggested that LPS-induced mediators such as IL-10 may be responsible for the observed anergy in patients. |
| تدمد: | 1528-0020 0006-4971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af8ee84b7011c84e05abc3b3485eeb3fTest https://doi.org/10.1182/blood.v91.9.3439Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af8ee84b7011c84e05abc3b3485eeb3f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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