Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR
| العنوان: | Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR |
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| المؤلفون: | Eva Malatinkova, Karen Vervisch, Pawel Bonczkowski, Bruno Verhasselt, Wim Trypsteen, Jolien Vermeire, Maja Kiselinova, Peter Messiaen, Ward De Spiegelaere, Linos Vandekerckhove |
| المصدر: | Journal of Clinical Microbiology. 53:699-701 |
| بيانات النشر: | American Society for Microbiology, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Microbiology (medical), viruses, Terminal Repeat Sequences, Human immunodeficiency virus (HIV), Biology, medicine.disease_cause, Polymerase Chain Reaction, Virology, DNA extraction, Long terminal repeat, law.invention, genomic DNA, Plasmid, Viral replication, law, DNA, Viral, HIV-1, medicine, Humans, Digital polymerase chain reaction, Polymerase chain reaction, Plasmids |
| الوصف: | Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation. |
| تدمد: | 1098-660X 0095-1137 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bef60234c45b80e50dd6cdeefe4be121Test https://doi.org/10.1128/jcm.03087-14Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bef60234c45b80e50dd6cdeefe4be121 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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