The LEM Domain Proteins Emerin and LAP2α Are Dispensable for Human Immunodeficiency Virus Type 1 and Murine Leukemia Virus Infections
| العنوان: | The LEM Domain Proteins Emerin and LAP2α Are Dispensable for Human Immunodeficiency Virus Type 1 and Murine Leukemia Virus Infections |
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| المؤلفون: | Roland Foisner, Colin L. Stewart, Vineet N. KewalRamani, Tatiana V. Cohen, Barbara Korbei, Serguei Kozlov, Alok Mulky |
| المصدر: | Journal of Virology. 82:5860-5868 |
| بيانات النشر: | American Society for Microbiology, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | viruses, Immunology, Emerin, Kidney, Microbiology, Virus, Cell Line, Mice, Transduction (genetics), Virology, Murine leukemia virus, Animals, Humans, Gene silencing, Cells, Cultured, Gammaretrovirus, Mice, Knockout, Infectivity, biology, Membrane Proteins, Nuclear Proteins, Fibroblasts, Embryo, Mammalian, biology.organism_classification, Virus-Cell Interactions, Protein Structure, Tertiary, DNA-Binding Proteins, Leukemia Virus, Murine, Insect Science, Knockout mouse, HIV-1, NIH 3T3 Cells, Retroviridae Infections |
| الوصف: | The human nuclear envelope proteins emerin and lamina-associated polypeptide 2α (LAP2α) have been proposed to aid in the early replication steps of human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MLV). However, whether these factors are essential for HIV-1 or MLV infection has been questioned. Prior studies in which conflicting results were obtained were highly dependent on RNA interference-mediated gene silencing. To shed light on these contradictory results, we examined whether HIV-1 or MLV could infect primary cells from mice deficient for emerin, LAP2α, or both emerin and LAP2α. We observed HIV-1 and MLV infectivity in mouse embryonic fibroblasts (MEFs) from emerin knockout, LAP2α knockout, or emerin and LAP2α double knockout mice to be comparable in infectivity to wild-type littermate-derived MEFs, indicating that both emerin and LAP2α were dispensable for HIV-1 and MLV infection of dividing, primary mouse cells. Because emerin has been suggested to be important for infection of human macrophages by HIV-1, we also examined HIV-1 transduction of macrophages from wild-type mice or knockout mice, but again we did not observe a difference in susceptibility. These findings prompted us to reexamine the role of human emerin in supporting HIV-1 and MLV infection. Notably, both viruses efficiently infected human cells expressing high levels of dominant-negative emerin. We thus conclude that emerin and LAP2α are not required for the early replication of HIV-1 and MLV in mouse or human cells. |
| تدمد: | 1098-5514 0022-538X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d11b01cfde7da7cdae623b59d2f1dbdbTest https://doi.org/10.1128/jvi.00076-08Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d11b01cfde7da7cdae623b59d2f1dbdb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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