التفاصيل البيبلوغرافية
| العنوان: |
Immunological activity of a 14-kilodalton recombinant protein of Mycobacterium tuberculosis H37Rv |
| المؤلفون: |
Kingston, A E, Salgame, P R, Mitchison, N A, Colston, M J |
| المصدر: |
Infection and Immunity ; volume 55, issue 12, page 3149-3154 ; ISSN 0019-9567 1098-5522 |
| بيانات النشر: |
American Society for Microbiology |
| سنة النشر: |
1987 |
| الوصف: |
A 14-kilodalton peptide antigen from Mycobacterium tuberculosis was isolated from an Escherichia coli lambda gt 11 recombinant DNA clone and was identified by Western blotting (immunoblotting) with monoclonal antibody TB68. Immunization of mice and guinea pigs with the recombinant peptide (rTB68) induced in vitro lymphoproliferative responses in draining lymph node lymphocyte cultures as well as in vivo delayed-type hypersensitivity reactions. Moreover, rTB68 was found both to induce and to cross-react with Mycobacterium leprae immune lymphocytes, but did not generate protective effects against live M. leprae challenge in mice. These findings showed that a 14-kilodalton peptide which has been characterized as specific for M. tuberculosis on the basis of B-cell recognition was capable of generating cell-mediated immune responses and moreover contained T-cell epitopes which were cross-reactive with M. leprae antigens. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1128/iai.55.12.3149-3154.1987 |
| الإتاحة: |
https://doi.org/10.1128/iai.55.12.3149-3154.1987Test |
| حقوق: |
https://journals.asm.org/non-commercial-tdm-licenseTest |
| رقم الانضمام: |
edsbas.3A85C4AC |
| قاعدة البيانات: |
BASE |
