rK39 Enzyme-Linked Immunosorbent Assay for Diagnosis of Leishmania donovani Infection
| العنوان: | rK39 Enzyme-Linked Immunosorbent Assay for Diagnosis of Leishmania donovani Infection |
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| المؤلفون: | Steven G. Reed, Eltahir A G Khalil, Ahmed M. Elhassan, Piet A. Kager, H. W. Ghalib, N. S. Daifalla, E.E. Zijlstra |
| المساهمون: | Other departments |
| المصدر: | Scopus-Elsevier Clinical and diagnostic laboratory immunology, 5(5), 717-720. American Society for Microbiology |
| بيانات النشر: | American Society for Microbiology, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Adult, Microbiology (medical), Adolescent, Clinical Biochemistry, Immunology, Protozoan Proteins, Leishmania donovani, Antibodies, Protozoan, Antigens, Protozoan, Enzyme-Linked Immunosorbent Assay, Article, Sudan, Immune system, Antigen, Seroepidemiologic Studies, Agglutination Tests, Direct agglutination test, mental disorders, parasitic diseases, medicine, Animals, Humans, Immunology and Allergy, Child, Amastigote, Subclinical infection, biology, Leishmaniasis, Middle Aged, biology.organism_classification, medicine.disease, Virology, Child, Preschool, biology.protein, Leishmaniasis, Visceral, Antibody |
| الوصف: | The rK39 enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for Leishmania donovani infection in the Sudan. rK39 ELISA proved more sensitive than DAT in diagnosis of kala-azar (93 and 80%, respectively); both tests may remain positive up to 24 months after treatment. For patients with post-kala-azar dermal leishmaniasis and individuals with subclinical infection, rK39 ELISA performed as well as DAT but could detect infection 6 months earlier in ∼40% of patients. Conversion in DAT and rK39 ELISA also occurred in leishmanin skin test (LST)-positive individuals, suggesting active parasite replication (rK39 is an amastigote antigen) in these presumably immune individuals. In contrast to DAT, rK39 ELISA also detected infection in randomly selected LST-positive individuals (in four of six) and endemicity (LST-negative) controls (in one of five). rK39 ELISA appears more sensitive than DAT and may prove an important tool in epidemiological studies. |
| تدمد: | 1098-6588 1071-412X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac2fb303be4d17bc8aafb0b336dddbd8Test https://doi.org/10.1128/cdli.5.5.717-720.1998Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac2fb303be4d17bc8aafb0b336dddbd8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10986588 1071412X |
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