Pseudomonas aeruginosa Triggers Macrophage Autophagy To Escape Intracellular Killing by Activation of the NLRP3 Inflammasome
| العنوان: | Pseudomonas aeruginosa Triggers Macrophage Autophagy To Escape Intracellular Killing by Activation of the NLRP3 Inflammasome |
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| المؤلفون: | Xi Huang, Minhao Wu, Meiyu Li, Yi Wang, Yongjian Wu, Dandan Li, Yuanqing Zhang, Qiuchan Deng, Jieying Pu |
| المصدر: | Infection and Immunity. 84:56-66 |
| بيانات النشر: | American Society for Microbiology, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Phagocyte, Interleukin-1beta, Immunology, Caspase 1, Inflammation, Ubiquitin-Activating Enzymes, Biology, Autophagy-Related Protein 7, Microbiology, Cell Line, Young Adult, 03 medical and health sciences, Phagocytosis, NLR Family, Pyrin Domain-Containing 3 Protein, Autophagy, medicine, Humans, Macrophage, Secretion, RNA, Small Interfering, Macrophages, Membrane Proteins, Inflammasome, Bacterial Infections, Cell biology, CARD Signaling Adaptor Proteins, Cytoskeletal Proteins, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Pseudomonas aeruginosa, Beclin-1, Female, RNA Interference, Parasitology, medicine.symptom, Apoptosis Regulatory Proteins, Carrier Proteins, Microtubule-Associated Proteins, medicine.drug |
| الوصف: | Assembly of the inflammasome has recently been identified to be a critical event in the initiation of inflammation. However, its role in bacterial killing remains unclear. Our study demonstrates that Pseudomonas aeruginosa infection induces the assembly of the NLRP3 inflammasome and the sequential secretion of caspase1 and interleukin-1β (IL-1β) in human macrophages. More importantly, activation of the NLRP3 inflammasome reduces the killing of P. aeruginosa in human macrophages, without affecting the generation of antimicrobial peptides, reactive oxygen species, and nitric oxide. In addition, our results demonstrate that P. aeruginosa infection increases the amount of the LC3-II protein and triggers the formation of autophagosomes in human macrophages. The P. aeruginosa -induced autophagy was enhanced by overexpression of NLRP3, ASC, or caspase1 but was reduced by knockdown of these core molecules of the NLRP3 inflammasome. Treatment with IL-1β enhanced autophagy in human macrophages. More importantly, IL-1β decreased the macrophage-mediated killing of P. aeruginosa , whereas knockdown of ATG7 or Beclin1 restored the IL-1β-mediated suppression of bacterial killing. Collectively, our study explores a novel mechanism employed by P. aeruginosa to escape from phagocyte killing and may provide a better understanding of the interaction between P. aeruginosa and host immune cells, including macrophages. |
| تدمد: | 1098-5522 0019-9567 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7880516cae30510116866fdf19496a82Test https://doi.org/10.1128/iai.00945-15Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7880516cae30510116866fdf19496a82 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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