Salmonella Paratyphi A Outer Membrane Vesicles Displaying Vi Polysaccharide as a Multivalent Vaccine against Enteric Fever
| العنوان: | Salmonella Paratyphi A Outer Membrane Vesicles Displaying Vi Polysaccharide as a Multivalent Vaccine against Enteric Fever |
|---|---|
| المؤلفون: | Maria Grazia Aruta, Renzo Alfini, Piero Mastroeni, Gianmarco Gasperini, Omar Rossi, Panchali Kanvatirth, Derek Pickard, Francesca Micoli, Francesca Necchi, Vanessa Arato, Francesca Mancini, Allan James Saul |
| المصدر: | Infection and Immunity. 89 |
| بيانات النشر: | American Society for Microbiology, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Serotype, Salmonella, Immunology, Salmonella paratyphi A, Biology, medicine.disease, medicine.disease_cause, Microbiology, Typhoid fever, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Infectious Diseases, Immune system, Antigen, medicine, biology.protein, Parasitology, 030212 general & internal medicine, Antibody, Bacterial outer membrane |
| الوصف: | Typhoid and paratyphoid fevers have a high incidence worldwide and coexist in many geographical areas, especially in low-middle-income countries (LMIC) in South and Southeast Asia. There is extensive consensus on the urgent need for better and affordable vaccines against systemic Salmonella infections. Generalized modules for membrane antigens (GMMA), outer membrane exosomes shed by Salmonella bacteria genetically manipulated to increase blebbing, resemble the bacterial surface where protective antigens are displayed in their native environment. Here, we engineered S. Paratyphi A using the pDC5-viaB plasmid to generate GMMA displaying the heterologous S. Typhi Vi antigen together with the homologous O:2 O antigen. The presence of both Vi and O:2 was confirmed by flow cytometry on bacterial cells, and their amount was quantified on the resulting vesicles through a panel of analytical methods. When tested in mice, such GMMA induced a strong antibody response against both Vi and O:2, and these antibodies were functional in a serum bactericidal assay. Our approach yielded a bivalent vaccine candidate able to induce immune responses against different Salmonella serovars, which could benefit LMIC residents and travelers. |
| تدمد: | 1098-5522 0019-9567 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::91e9177be26e2d1dff7e4e4230d95483Test https://doi.org/10.1128/iai.00699-20Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........91e9177be26e2d1dff7e4e4230d95483 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
|---|