التفاصيل البيبلوغرافية
العنوان: |
A comparative analysis of FISH, RT-PCR, and cytogenetics for the diagnosis of bcr-abl-positive leukemias |
المؤلفون: |
Cox, M, Maffei, L, Buffolino, S, Aronica, G, Aquilina, P, Masi, M, DEL POETA, GIOVANNI, VENDITTI, ADRIANO, CANTONETTI, MARIA, AMADORI, SERGIO |
المساهمون: |
Cox, M, Maffei, L, Buffolino, S, DEL POETA, G, Venditti, A, Cantonetti, M, Aronica, G, Aquilina, P, Masi, M, Amadori, S |
بيانات النشر: |
American Society for Clinical Pathology |
سنة النشر: |
1998 |
المجموعة: |
Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
مصطلحات موضوعية: |
bcr-abl gene translocation, Fluorescence in situ hybridization [FISH], Leukemia diagnosi, Ph-positive leukemias, Settore MED/15 - MALATTIE DEL SANGUE, Settore MED/06 - ONCOLOGIA MEDICA |
الوصف: |
Philadelphia (Ph) chromosome-positive leukemias, with the bcr-abl gene translocation, have a dismal prognosis. The identification of Ph-positive patients is vitally important because only aggressive therapeutic approaches, such as allogeneic bone marrow transplantation, may result in long-term disease-free survival. Routine diagnostic methods, such as Southern blot analysis and cytogenetics, may lead to false-negative results. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis is considered the most sensitive tool for the detection of the bcr-abl translocation, and it is widely used alone or in combination with karyotyping or Southern blot analysis to identify Ph-positive cases. In this study, we used fluorescence in situ hybridization (FISH) with BCR and ABL double-color probes for detecting Ph-positive leukemias. The FISH results were compared with the results of cytogenetic and RT-PCR analyses in 75 patients with leukemia or other myeloproliferative syndromes (chronic myeloid leukemia, 30; acute lymphoblastic leukemia, 24; acute myelogenous leukemia, 6; essential (hemorrhagic) thrombocythemia, 12; chronic myelomonocytic leukemia, 2; and polycythemia vera, 1). FISH analysis proved to be simple, extremely reliable and sensitive; bcr-abl fusion detection was successful in the presence of all types of molecular junctions ie, (b2a2, b3a2, and e1a2). Furthermore, a Ph- positive case that proved fusion negative by RT-PCR was identified as positive by FISH. The sensitivity of RT-PCR and FISH related to Ph-positive cases were 97% and 100%, respectively. Regarding specificity, in 4 (5%) of 75 patients, RT-PCR provided false-positive results. Cross-contamination was identified because a new specimen was harvested and reanalyzed when FISH, cytogenetics, and RT-PCR results were contradictory. We believe FISH is an optimal diagnostic method to detect bcr-abl translocation that can be used alone or to validate the results of RT-PCR analysis. |
نوع الوثيقة: |
article in journal/newspaper |
اللغة: |
English |
العلاقة: |
info:eu-repo/semantics/altIdentifier/pmid/9426514; info:eu-repo/semantics/altIdentifier/wos/WOS:000071185200006; volume:109; issue:1; firstpage:24; lastpage:31; numberofpages:8; journal:AMERICAN JOURNAL OF CLINICAL PATHOLOGY; http://hdl.handle.net/2108/69290Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0031983562 |
الإتاحة: |
http://hdl.handle.net/2108/69290Test |
رقم الانضمام: |
edsbas.A9FA7E87 |
قاعدة البيانات: |
BASE |