Crohn disease–associated adherent-invasive E. coli bacteria target mouse and human Peyer’s patches via long polar fimbriae
| العنوان: | Crohn disease–associated adherent-invasive E. coli bacteria target mouse and human Peyer’s patches via long polar fimbriae |
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| المؤلفون: | Amélie de Vallée, Arlette Darfeuille-Michaud, Benoit Chassaing, Maelle Prorok-Hamon, Sa’ad Y. Salim, Christel Neut, Johan D. Söderholm, Jean-Pierre Hugot, Jean-Frederic Colombel, Barry J. Campbell, Nathalie Rolhion |
| المساهمون: | Institut National de la Recherche Agronomique (INRA), Institut Universitaire de Technologie de Clermont Ferrand, Linköping University (LIU), University of Liverpool, Université de Lille, Droit et Santé, Université Paris Diderot - Paris 7 (UPD7), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Ministere de la Recherche et de la Technologie [JE2526], INRA [USC-2018], Association F. Aupetit (AFA), European Commission, Swedish Research Council [VR-M 2005-7013], National Institute of Health Research Specialist Biomedical Research Centre in Microbial Disease [01CD1], Wellcome Trust UK [074949/Z/04/Z], National Association for Colitis and Crohn's Disease UK [M/08/1] |
| المصدر: | Journal of Clinical Investigation Journal of Clinical Investigation, American Society for Clinical Investigation, 2011, 121 (3), pp.966-975. ⟨10.1172/JCI44632⟩ |
| بيانات النشر: | American Society for Clinical Investigation, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], Fimbria, Nod2 Signaling Adaptor Protein, medicine.disease_cause, Bacterial Adhesion, Epithelium, Mice, Peyer's Patches, 0302 clinical medicine, Crohn Disease, Intestine, Small, health care economics and organizations, Microfold cell, 0303 health sciences, Microscopy, Confocal, General Medicine, 3. Good health, medicine.anatomical_structure, ESCHERICHIA-COLI, 030211 gastroenterology & hepatology, Research Article, EXPRESSION, education, Virulence, Ileum, Mice, Transgenic, Biology, PATIENT, Microbiology, 03 medical and health sciences, Immune system, TYPE-1 PILI, medicine, Escherichia coli, Animals, Humans, FOLLICLE-ASSOCIATED EPITHELIUM, M-CELL MODEL, 030304 developmental biology, Inflammation, ILEAL MUCOSA, STRAIN LF82, SALMONELLA-TYPHIMURIUM, Small intestine, Fimbriae, Bacterial, Immune System, Mutation, INFLAMMATORY-BOWEL-DISEASE |
| الوصف: | International audience; Crohn disease (CD) is a multifactorial disease in which an abnormal immune response in the gastrointestinal (GI) tract leads to chronic inflammation. The small intestine, particularly the ileum, of patients with CD is colonized by adherent-invasive E. coil (AIEC) a pathogenic group of E. coil able to adhere to and invade intestinal epithelial cells. As the earliest inflammatory lesions are microscopic erosions of the epithelium lining the Peyer's patches (PPs), we investigated the ability of AIEC bacteria to interact with PPs and the virulence factors involved. We found that AIEC bacteria could interact with mouse and human PPs via long polar fimbriae (LPF). An LPF-negative AIEC mutant was highly impaired in its ability to interact with mouse and human PPs and to translocate across monolayers of M cells, specialized epithelial cells at the surface of PPs. The prevalence of AIEC strains harboring the lpf operon was markedly higher in CD patients compared with controls. In addition, increased numbers of AIEC, but not LPF-deficient AIEC, bacteria were found interacting with PPs from Nod2(-/-) mice compared with WT mice. In conclusion, we have identified LPF as a key factor for AIEC to target PPs. This could be the missing link between AIEC colonization and the presence of early lesions in the PPs of CD patients. |
| اللغة: | English |
| تدمد: | 0021-9738 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70618ca181bbc6bddd38b9eb50ad5604Test https://europepmc.org/articles/PMC3049390Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....70618ca181bbc6bddd38b9eb50ad5604 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219738 |
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