ER exit sites are physical and functional core autophagosome biogenesis components
| العنوان: | ER exit sites are physical and functional core autophagosome biogenesis components |
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| المؤلفون: | Martin Graef, Jonathan R. Friedman, Mohan Babu, Jodi Nunnari, Chris Graham |
| المصدر: | Molecular Biology of the Cell |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Autophagosome, Saccharomyces cerevisiae, Vacuole, Biology, Endoplasmic Reticulum, Models, Biological, 03 medical and health sciences, 0302 clinical medicine, Phagosomes, Lysosome, Chlorocebus aethiops, Autophagy, medicine, Animals, Molecular Biology, COPII, 030304 developmental biology, 0303 health sciences, Endoplasmic reticulum, Articles, Cell Biology, COP-Coated Vesicles, Cell biology, medicine.anatomical_structure, Membrane Trafficking, COS Cells, Vacuoles, hormones, hormone substitutes, and hormone antagonists, 030217 neurology & neurosurgery, Biogenesis, Protein Binding |
| الوصف: | ERES function is required for assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex and is associated with formation of autophagosomes at every stage of the process. ERES are core components of the autophagy machinery for the biogenesis of autophagosomes. Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites. |
| تدمد: | 1939-4586 1059-1524 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47b200292a04214535e97c6d97297a04Test https://doi.org/10.1091/mbc.e13-07-0381Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....47b200292a04214535e97c6d97297a04 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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