Control of Bro1-Domain Protein Rim20 Localization by External pH, ESCRT Machinery, and theSaccharomyces cerevisiaeRim101 Pathway
| العنوان: | Control of Bro1-Domain Protein Rim20 Localization by External pH, ESCRT Machinery, and theSaccharomyces cerevisiaeRim101 Pathway |
|---|---|
| المؤلفون: | Jacob H. Boysen, Aaron P. Mitchell |
| المصدر: | Molecular Biology of the Cell. 17:1344-1353 |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, Sodium-Hydrogen Exchangers, Endosome, Recombinant Fusion Proteins, Protein subunit, Protein domain, Saccharomyces cerevisiae, Vesicular Transport Proteins, macromolecular substances, Models, Biological, ESCRT, Transport Vesicles, Cation Transport Proteins, Molecular Biology, Endosomal Sorting Complexes Required for Transport, biology, Nuclear Proteins, Articles, Cell Biology, Hydrogen-Ion Concentration, biology.organism_classification, Endocytosis, Protein Structure, Tertiary, Cell biology, Transport protein, DNA-Binding Proteins, Repressor Proteins, ESCRT complex, VPS25, Protein Transport, Mutation, Vacuoles |
| الوصف: | Bro1-domain proteins such as yeast Bro1 and mammalian AIP1/Alix are well-established participants in endosome metabolism. The Bro1-domain interacts with endosomal surface protein Snf7/Vps32 in yeast, a subunit of the ESCRT complex. Yeast Bro1-domain protein Rim20 has no role in endosome function, but is required for alkaline pH-stimulated cleavage of transcription factor Rim101. Rim20-GFP is cytoplasmic under acidic conditions but concentrated in punctate foci under alkaline conditions. Bro1-GFP also accumulates in foci, but they are more numerous under acidic than alkaline conditions. Colocalization experiments indicate that some Rim20-GFP foci correspond to Bro1-RFP foci, whereas others do not. Rim8, Rim9, Rim21, Dfg16, Snf7, Vps20, Vps23, and Vps25, which are required for Rim101 cleavage, are required for appearance of Rim20-GFP foci. ESCRT complexes accumulate on endosome-derived compartments in cells that lack the AAA-ATPase Vps4. We find that Rim20-GFP foci accumulate in a vps4 mutant background independently of external pH, Rim101 pathway-specific genes, and most ESCRT subunit genes except for SNF7. Rim20-GFP foci seem to represent endosomes, because they colocalize with Snf7-RFP and because they correspond to a perivacuolar compartment in the vps4 strain. We propose that alkaline growth conditions alter the endosomal surface to favor Rim20-Snf7 interaction and Rim101 cleavage. Our findings raise the possibility that Bro1-domain proteins may be differentially regulated in the same cell, thereby coupling endosome metabolism to signaling. |
| تدمد: | 1939-4586 1059-1524 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b7ecfcfc862da20dc7bc733bf52174dTest https://doi.org/10.1091/mbc.e05-10-0949Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5b7ecfcfc862da20dc7bc733bf52174d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
|---|