Microelectrode measurements of pericellular PO2 in erythropoietin-producing human hepatoma cell cultures
| العنوان: | Microelectrode measurements of pericellular PO2 in erythropoietin-producing human hepatoma cell cultures |
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| المؤلفون: | Joachim Fandrey, Wolfgang Jelkmann, Martin Wolff |
| المصدر: | American Journal of Physiology-Cell Physiology. 265:C1266-C1270 |
| بيانات النشر: | American Physiological Society, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Carcinoma, Hepatocellular, Time Factors, Physiology, Partial Pressure, Biology, Cell Line, Oxygen Consumption, Culture Techniques, Tumor Cells, Cultured, medicine, Humans, Erythropoietin, Liver Neoplasms, Cell Biology, Cell biology, Oxygen, Kinetics, Microelectrode, Glucose, Biochemistry, Established cell line, Cell culture, Lactates, cardiovascular system, Extracellular Space, Hepatoma cell, Microelectrodes, medicine.drug |
| الوصف: | On the basis of Fick's law of gas diffusion, it has been proposed that cells in conventional monolayer cultures may be severely hypoxic. Because knowledge of the cellular O2 availability is important for the interpretation of biochemical and toxicological cell culture work, microelectrode measurements of the pericellular PO2 were carried out using the erythropoietin (Epo)-producing human hepatoma cell lines Hep G2 and Hep 3B as an in vitro model. In confluent hepatoma cultures grown in polystyrene dishes and incubated in air with 5% CO2, the pericellular steady-state PO2 was < 1 mmHg. The rates of the production of immunoreactive Epo and lactate were high due to a misproportion between O2 supply and O2 requirements. Epo production decreased when shaken instead of static cultures were studied, or when the O2 concentration in the gas atmosphere was increased gradually up to 95%. In cultures grown on gas-permeable supports, pericellular and gas PO2 values were very similar, with increased Epo production at lowered PO2. In agreement with mathematical models, our experimental data make PO2 measurements desirable for studies of O2-dependent biological functions in cell cultures. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6627c2152336d32438996020dbe71bf2Test https://doi.org/10.1152/ajpcell.1993.265.5.c1266Test |
| رقم الانضمام: | edsair.doi.dedup.....6627c2152336d32438996020dbe71bf2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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