NHE2 and NHE3 are human and rabbit intestinal brush-border proteins
| العنوان: | NHE2 and NHE3 are human and rabbit intestinal brush-border proteins |
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| المؤلفون: | Patricia D. Wilson, W. A. Hoogerwerf, Chung-Ming Tse, S. C. Tsao, C. Chris Yun, Mark Donowitz, Susan A. Levine, Jeannie W. Yip, Michael Cohen, A. J. Lazenby, Olivier Devuyst |
| المصدر: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 270:G29-G41 |
| بيانات النشر: | American Physiological Society, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Male, Sodium-Hydrogen Exchangers, Brush border, Physiology, Blotting, Western, Molecular Sequence Data, Crypt, Ileum, Biology, Kidney, digestive system, Descending colon, Jejunum, Physiology (medical), Intestine, Small, medicine, Animals, Humans, Ascending colon, Tissue Distribution, Epithelial polarity, Base Sequence, Microvilli, Hepatology, urogenital system, digestive, oral, and skin physiology, Gastroenterology, Immunohistochemistry, Molecular biology, digestive system diseases, Small intestine, medicine.anatomical_structure, Biochemistry, Molecular Probes, Rabbits |
| الوصف: | Rabbit NHE2 and NHE3 are two epithelial isoform Na+/H+ exchangers (NHE), the messages for which are found predominantly and entirely, respectively, in renal, intestinal, and gastric mucosa. The current studies used Western analysis and immunohistochemistry to identify and characterize the apical vs. basolateral membrane distribution of NHE2 and NHE3 in intestinal epithelial cells. Based on Western analysis, NHE2 and NHE3 both are present in brush-border but not basolateral membranes of small intestine. Both NHE2 and NHE3 are 85-kDa proteins. Consistent with Western analysis, NHE2 and NHE3 are immunolocalired to the brush-border but not basolateral membranes of villus epithelial cells, but not goblet cells, in human jejunum and ileum and in surface epithelial cells in the ascending and descending colon and rectum. In addition, NHE2 and NHE3 are present in small amounts in the crypt cell brush border of human jejunum, ileum, ascending and descending colon, and rectum. In rabbit jejunum, ileum, and ascending colon, NHE2 and NHE3 are present in the brush border of epithelial and not goblet cells, again much more in the villus (small intestine)/ surface cells (colon) than the crypt. NHE2 but not NHE3 is present in the brush border of rabbit descending colon surface cells and in small amounts in crypt cells. NHE2 and NHE3 are both human and rabbit small intestinal and colonic epithelial cell brush-border Na+/H+ exchanger isoforms that colocalize in all intestinal segments except rabbit descending colon, which lacks NHE3. |
| تدمد: | 1522-1547 0193-1857 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c152e13baab63eeb01f18644dfe60472Test https://doi.org/10.1152/ajpgi.1996.270.1.g29Test |
| رقم الانضمام: | edsair.doi.dedup.....c152e13baab63eeb01f18644dfe60472 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221547 01931857 |
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