التفاصيل البيبلوغرافية
| العنوان: |
Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein. |
| المؤلفون: |
Akemann, Walther, Mutoh, Hiroki, Perron, Amélie, Park, Yun Kyung, Iwamoto, Yuka, Knöpfel, Thomas |
| المصدر: |
Journal of Neurophysiology; Oct2012, Vol. 108 Issue 8, p2323-2337, 15p |
| مصطلحات موضوعية: |
NEURAL circuitry, BRAIN imaging, FLUORESCENT proteins, ELECTRIC potential, ELECTROENCEPHALOGRAPHY, MEMBRANE potential |
| مستخلص: |
Population signals from neuronal ensembles in cortex during behavior are commonly measured with EEG, local field potential (LFP), and voltage-sensitive dyes. A genetically encoded voltage indicator would be useful for detection of such signals in specific cell types. Here we describe how this goal can be achieved with Butterfly, a voltage-sensitive fluorescent protein (VSFP) with a subthreshold detection range and enhancements designed for voltage imaging from single neurons to brain in vivo. VSFP-Butterfly showed reliable membrane targeting, maximum response gain around standard neuronal resting membrane potential, fast kinetics for single-cell synaptic responses, and a high signal-to-noise ratio. Butterfly reports excitatory postsynaptic potentials (EPSPs) in cortical neurons, whisker-evoked responses in barrel cortex, 25-Hz gamma oscillations in hippocampal slices, and 2- to 12-Hz slow waves during brain state modulation in vivo. Our findings demonstrate that cell class-specific voltage imaging is practical with VSFP-Butterfly, and expand the genetic toolbox for the detection of neuronal population dynamics. [ABSTRACT FROM AUTHOR] |
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