Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells?
| العنوان: | Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells? |
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| المؤلفون: | Ning Wang, Donald E. Ingber, D. Stamenovic, Andrew Maniotis, Jacob Pourati, James P. Butler, Jeffrey J. Fredberg, Jonathan L. Schaffer, David S. Spiegel |
| المصدر: | American Journal of Physiology-Cell Physiology. 274:C1283-C1289 |
| بيانات النشر: | American Physiological Society, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Endothelium, Physiology, Cell, Cell Biology, Elasticity, Endothelial stem cell, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Cell culture, Immunology, Cell Adhesion, medicine, Biophysics, Stress, Mechanical, Cytoskeleton, Cell adhesion, Actin, Cytochalasin D |
| الوصف: | We tested the hypothesis that mechanical tension in the cytoskeleton (CSK) is a major determinant of cell deformability. To confirm that tension was present in adherent endothelial cells, we either cut or detached them from their basal surface by a microneedle. After cutting or detachment, the cells rapidly retracted. This retraction was prevented, however, if the CSK actin lattice was disrupted by cytochalasin D (Cyto D). These results confirmed that there was preexisting CSK tension in these cells and that the actin lattice was a primary stress-bearing component of the CSK. Second, to determine the extent to which that preexisting CSK tension could alter cell deformability, we developed a stretchable cell culture membrane system to impose a rapid mechanical distension (and presumably a rapid increase in CSK tension) on adherent endothelial cells. Altered cell deformability was quantitated as the shear stiffness measured by magnetic twisting cytometry. When membrane strain increased 2.5 or 5%, the cell stiffness increased 15 and 30%, respectively. Disruption of actin lattice with Cyto D abolished this stretch-induced increase in stiffness, demonstrating that the increased stiffness depended on the integrity of the actin CSK. Permeabilizing the cells with saponin and washing away ATP and Ca2+did not inhibit the stretch-induced stiffening of the cell. These results suggest that the stretch-induced stiffening was primarily due to the direct mechanical changes in the forces distending the CSK but not to ATP- or Ca2+-dependent processes. Taken together, these results suggest preexisting CSK tension is a major determinant of cell deformability in adherent endothelial cells. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79c531cb8a0a25d97246f9391d6df7e0Test https://doi.org/10.1152/ajpcell.1998.274.5.c1283Test |
| رقم الانضمام: | edsair.doi.dedup.....79c531cb8a0a25d97246f9391d6df7e0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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