In vitro cell cultures of Brunner’s glands from male mouse to study GLP-1 receptor function
| العنوان: | In vitro cell cultures of Brunner’s glands from male mouse to study GLP-1 receptor function |
|---|---|
| المؤلفون: | Louise M. Voetmann, Christina R. Underwood, Bidda Rolin, Axel K. Hansen, Rikke K. Kirk, Charles Pyke, Lotte B. Knudsen, Klaus S. Frederiksen |
| المصدر: | Voetmann, L M, Underwood, C R, Rolin, B, Hansen, A K, Kirk, R K, Pyke, C, Knudsen, L B & Frederiksen, K S 2022, ' In vitro cell cultures of Brunner's glands from male mouse to study GLP-1 receptor function ', American journal of physiology. Cell physiology, vol. 322, no. 6, pp. C1260-C1269 . https://doi.org/10.1152/ajpcell.00345.2021Test |
| بيانات النشر: | American Physiological Society, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Male, Duodenum, Physiology, digestive, oral, and skin physiology, Cell Culture Techniques, in vitro, Brunner Glands, Brunner’s glands, Cell Biology, digestive system, Glucagon-Like Peptide-1 Receptor, Mice, Mucus, stomatognathic system, mucus, gut, Animals, Intestinal Mucosa, GLP-1, mouse |
| الوصف: | Exocrine glands in the submucosa of the proximal duodenum secrete alkaline fluid containing mucus to protect the intestinal mucosa from acidic stomach contents. These glands, known as Brunner’s glands, express high glucagon-like peptide 1 receptor (GLP-1R) levels. Previous studies have suggested that activation of the GLP-1R induces expression of barrier protective genes in Brunner’s glands. Still, the lack of a viable in vitro culture of Brunner’s glands has hampered additional studies of the functional consequences of GLP-1R activation. In this study, we established a procedure to isolate and culture cells derived from murine Brunner’s glands. The isolated glandular cells retained functional GLP-1R expression in culture, making this in vitro system suitable for the study of GLP-1R activation. We found that cells derived from the Brunner’s glands of mice pretreated with semaglutide contained significantly more mucus compared with Brunner’s glands from vehicle-treated mice. Our data suggest a protective intestinal response upon semaglutide treatment, but further studies are required to leverage the full potential of cultured Brunner’s gland cells. |
| وصف الملف: | application/pdf |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d56cc99bef32b6101469030ddfc5a830Test https://doi.org/10.1152/ajpcell.00345.2021Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d56cc99bef32b6101469030ddfc5a830 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
|---|