Role of α1-adrenoceptor subtypes on corneal epithelial thickness and cell proliferation in mice
| العنوان: | Role of α1-adrenoceptor subtypes on corneal epithelial thickness and cell proliferation in mice |
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| المؤلفون: | Katharina Bell, Norbert Pfeiffer, Aytan Musayeva, Christoph Brochhausen, Adrian Gericke, Caroline Manicam, Beate K. Straub, Andreas Steege |
| المصدر: | American Journal of Physiology-Cell Physiology. 315:C757-C765 |
| بيانات النشر: | American Physiological Society, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Physiology, Chemistry, Cell growth, Adrenergic, Cell Biology, α1 adrenoceptor, Cell biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, 030221 ophthalmology & optometry, medicine, α adrenoceptors, Corneal epithelium |
| الوصف: | Adrenergic stimuli are important for corneal epithelial structure and healing. The purpose of the present study was to examine the hypothesis that the lack of a single α1-adrenoceptor (α1-AR) subtype affects corneal epithelial thickness and cell proliferation. Expression levels of α1-AR mRNA were determined in mouse cornea using real-time PCR. In mice devoid of one of the three α1-AR subtypes (α1A-AR−/−, α1B-AR−/−, α1D-AR−/−) and in wild-type controls, thickness of individual corneal layers, the number of epithelial cell layers, and average epithelial cell size were determined in cryosections. Endothelial cell density and morphology were calculated in corneal explants, and epithelial cell proliferation rate was determined with immunofluorescence microscopy. Moreover, the ultrastructure of the corneal epithelium was examined by transmission electron microscopy. Messenger RNA for all three α1-AR subtypes was expressed in whole cornea and in corneal epithelium from wild-type mice with a rank order of abundance of α1A≥ α1B> α1D. In contrast, no α1-AR mRNA was detected in the stroma, and only α1B-AR mRNA was found in the Descemet endothelial complex. Remarkably, corneal epithelial thickness and mean epithelial cell size were reduced in α1A-AR−/−mice. Our findings suggest that the α1A-AR exerts growth effects in mouse corneal epithelial cells. |
| تدمد: | 1522-1563 0363-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e9b4af3981b09df6bc0ccd7cec46c66cTest https://doi.org/10.1152/ajpcell.00314.2018Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........e9b4af3981b09df6bc0ccd7cec46c66c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221563 03636143 |
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