Cigarette smoke disrupts VEGF165-VEGFR-2 receptor signaling complex in rat lungs and patients with COPD: morphological impact of VEGFR-2 inhibition
| العنوان: | Cigarette smoke disrupts VEGF165-VEGFR-2 receptor signaling complex in rat lungs and patients with COPD: morphological impact of VEGFR-2 inhibition |
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| المؤلفون: | John A. Marwick, Irfan Rahman, Paul Kirkham, June Giddings, K D Butler, William MacNee, Christopher S. Stevenson |
| المصدر: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 290:L897-L908 |
| بيانات النشر: | American Physiological Society, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, Vascular Endothelial Growth Factor A, Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, Physiology, VEGF receptors, Rats, Sprague-Dawley, Pulmonary Disease, Chronic Obstructive, Forced Expiratory Volume, Smoke, Physiology (medical), Neuropilin 1, medicine, Animals, Humans, Cigarette smoke, Lung, COPD, biology, business.industry, Smoking, Receptor signaling, Kinase insert domain receptor, Cell Biology, medicine.disease, Vascular Endothelial Growth Factor Receptor-2, Rats, respiratory tract diseases, Cancer research, biology.protein, business, Signal Transduction |
| الوصف: | VEGF is fundamental in the development and maintenance of the vasculature. VEGF165signaling through VEGF receptor (VEGFR)-2/kinase insert domain receptor (KDR) is a highly regulated process involving the formation of a tertiary complex with glypican (GYP)-1 and neuropilin (NRP)-1. Both VEGF and VEGFR-2 expression are reduced in emphysematous lungs; however, the mechanism of regulation of VEGF165signaling through the VEGFR-2 complex in response to cigarette smoke exposure in vivo, and in smokers with and without chronic obstructive pulmonary disease (COPD), is still unknown. We hypothesized that cigarette smoke exposure disrupts the VEGF165-VEGFR-2 complex, a potential mechanism in the pathogenesis of emphysema. We show that cigarette smoke exposure reduces NRP-1 and GYP-1 as well as VEGF and VEGFR-2 levels in rat lungs and that VEGF, VEGFR-2, GYP-1, and NRP-1 expression in the lungs of both smokers and patients with COPD are also reduced compared with nonsmokers. Moreover, our data suggest that specific inhibition of VEGFR-2 alone with NVP-AAD777 would appear not to result in emphysema in the adult rat lung. As both VEGF165and VEGFR-2 expression are reduced in emphysematous lungs, decreased GYP-1 and NRP-1 expression may yet further disrupt VEGF165-VEGFR-2 signaling. Whether or not this by itself is critical for inducing endothelial cell apoptosis and decreased vascularization of the lung seen in emphysema patients is still unclear at present. However, targeted therapies to restore VEGF165-VEGFR-2 complex may promote endothelial cell survival and help to ameliorate emphysema. |
| تدمد: | 1522-1504 1040-0605 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::894124fa2429fcd57a724bd2fd086755Test https://doi.org/10.1152/ajplung.00116.2005Test |
| رقم الانضمام: | edsair.doi.dedup.....894124fa2429fcd57a724bd2fd086755 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
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