Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis
| العنوان: | Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis |
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| المؤلفون: | Lishuang Shen, Jonathan Cardona-Velez, Dhanesh Amarnani, Patricia A. D'Amore, Cindy Park-Windhol, Dean Eliott, Diane R. Bielenberg, Lindsay L. Wong, Daniel J. Oh, Tavé van Zyl, Xiaowu Gai, Jonathan D. Lam, Angie V. Sanchez, Declan McGuone, Joseph F. Arboleda-Velasquez, Leo A. Kim, Anat Stemmer-Rachamimov |
| المصدر: | Diabetes |
| بيانات النشر: | American Diabetes Association, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, CD31, Adult, Male, Angiogenesis, Endocrinology, Diabetes and Metabolism, Biology, Retinal Neovascularization, Pathophysiology, Retina, 03 medical and health sciences, chemistry.chemical_compound, Mice, Downregulation and upregulation, Cell Movement, hemic and lymphatic diseases, Internal Medicine, medicine, Animals, Humans, RNA, Messenger, Aged, Cell Proliferation, Tube formation, Aged, 80 and over, Diabetic Retinopathy, Endothelial Cells, Retinal, Diabetic retinopathy, Middle Aged, medicine.disease, Immunohistochemistry, Oxygen, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Diabetes Mellitus, Type 1, Glucose, chemistry, Diabetes Mellitus, Type 2, embryonic structures, Core Binding Factor Alpha 2 Subunit, Cancer research, Female, Retinopathy |
| الوصف: | Proliferative diabetic retinopathy (PDR) is a common cause of blindness in the developed world’s working adult population and affects those with type 1 and type 2 diabetes. We identified Runt-related transcription factor 1 (RUNX1) as a gene upregulated in CD31+ vascular endothelial cells obtained from human PDR fibrovascular membranes (FVMs) via transcriptomic analysis. In vitro studies using human retinal microvascular endothelial cells (HRMECs) showed increased RUNX1 RNA and protein expression in response to high glucose, whereas RUNX1 inhibition reduced HRMEC migration, proliferation, and tube formation. Immunohistochemical staining for RUNX1 showed reactivity in vessels of patient-derived FVMs and angiogenic tufts in the retina of mice with oxygen-induced retinopathy, suggesting that RUNX1 upregulation is a hallmark of aberrant retinal angiogenesis. Inhibition of RUNX1 activity with the Ro5–3335 small molecule resulted in a significant reduction of neovascular tufts in oxygen-induced retinopathy, supporting the feasibility of targeting RUNX1 in aberrant retinal angiogenesis. |
| اللغة: | English |
| تدمد: | 1939-327X 0012-1797 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::995b91f72944a437dd51ca30e73bba87Test http://europepmc.org/articles/PMC5482092Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....995b91f72944a437dd51ca30e73bba87 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1939327X 00121797 |
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