Human Plasma Copper Proteins Speciation by Size Exclusion Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry. Solutions for Columns Calibration by Sulfur Detection
| العنوان: | Human Plasma Copper Proteins Speciation by Size Exclusion Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry. Solutions for Columns Calibration by Sulfur Detection |
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| المؤلفون: | Souleiman El Balkhi, Joël Poupon, Jean-Marc Trocello, France Massicot, France Woimant, Olivier Laprévote |
| المصدر: | Analytical Chemistry. 82:6904-6910 |
| بيانات النشر: | American Chemical Society (ACS), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Chromatography, Argon, Mass-to-charge ratio, Chemistry, Copper protein, Size-exclusion chromatography, Analytical chemistry, Ceruloplasmin, chemistry.chemical_element, Plasma, Mass spectrometry, Mass Spectrometry, Analytical Chemistry, Oxygen, Gel permeation chromatography, Calibration, Metalloproteins, Chromatography, Gel, Humans, Carrier Proteins, Inductively coupled plasma mass spectrometry, Copper, Serum Albumin, Sulfur |
| الوصف: | Among the hyphenated techniques used to probe and identify metalloproteins, size exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS) has shown to have a central place. However, the calibration of SEC columns reveals to be tedious and always involves UV detection prior to ICP-MS. The presence of sulfur in 98% of proteins allows their detection by quadrupole ICP-MS, despite the isobaric interference ((16)O(16)O) on S, by monitoring (32)S(16)O at mass to charge ratio (m/z) 48. The formation of SO occurs spontaneously in the argon plasma but can be optimized by the introduction of oxygen gas into a reaction cell (RC) to achieve nM levels. In this article, sulfur detection was discussed upon instrumental conditions and S detection was then optimized by applying O(2) as a reaction gas. SO formation was used to calibrate SEC columns without UV detection. This simple SEC-ICP-MS method was used for plasma copper proteins in plasma healthy subjects (HS) and an untreated Wilson disease (WD) patient. Copper proteins identified in healthy subjects were transcuprein, ceruloplasmin (Cp) and albumin. The method led to results in good agreement with other methods of determination. Copper bound to Cp in the WD patient was lowered with regard to the HS, and the exchangeable Cu was highly increased. |
| تدمد: | 1520-6882 0003-2700 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb61cec0191321ef2045e62a01b7070fTest https://doi.org/10.1021/ac101128xTest |
| رقم الانضمام: | edsair.doi.dedup.....fb61cec0191321ef2045e62a01b7070f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206882 00032700 |
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