Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn
| العنوان: | Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn |
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| المؤلفون: | Jifei Yang, Gaiping Zhang, Xiaofei Hu, Yaning Sun, Ruiguang Deng, Yong Zhang, Wang Yao, Fangyu Wang |
| المصدر: | Journal of Agricultural and Food Chemistry. 62:11116-11121 |
| بيانات النشر: | American Chemical Society (ACS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Immunogen, Chromatography, biology, medicine.diagnostic_test, medicine.drug_class, Chemistry, fungi, Food Contamination, General Chemistry, Monoclonal antibody, Zea mays, Antibodies, Chromatography, Affinity, Titer, chemistry.chemical_compound, Immunoassay, biology.protein, medicine, Zearalenone, Zearalanone, Antibody, Bovine serum albumin, General Agricultural and Biological Sciences |
| الوصف: | A rapid immunochromatographic test strip has been developed for the detection of zearalenone (ZEN) residues in corn. For this purpose, a specific anti-ZEN monoclonal antibody (mAb), 4A3-F9, was obtained and identified. ZEN coupled to bovine serum albumin (BSA) via 1,4-butanediol diglycidyl ether was prepared as immunogen. The mAb showed low cross-reactivity with five ZEN analogues. Using an antibody preparation with a titer of ≥1:5.12 × 10(5), the cross-reactivity (CR) of the anti-ZEN monoclonal antibody with four of the analogues was4%, except for zearalanone, which was 53.121%. The recovery rates of ZEN in spiked corn samples were in the range of 91.30-97.07% with coefficients of variation5.32%. An immunochromatographic strip was developed using the specific anti-ZEN monoclonal antibody and applied to the screening of corn samples for ZEN residues. The test could be accomplished within 5-10 min. The sensitivity of the test strip in corn sample extract was confirmed to be 20 μg/kg by unaided visual assessment, and the IC50 was calculated as 3.4 ng/mL using a test strip reader. The test strip, analyzed by unaided visual assessment and strip reader, showed very good agreement with competitive indirect ELISA and high-performance liquid chromatography (HPLC) analysis for naturally contaminated corn samples. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::893463a85137160eb5101232490796a7Test https://doi.org/10.1021/jf503092jTest |
| رقم الانضمام: | edsair.doi.dedup.....893463a85137160eb5101232490796a7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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