Rat Coagulating Gland Secretion Contains a Kinesin Heavy Chain-like Protein Acting as a Type IV Transglutaminase Substrate
| العنوان: | Rat Coagulating Gland Secretion Contains a Kinesin Heavy Chain-like Protein Acting as a Type IV Transglutaminase Substrate |
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| المؤلفون: | Stefania Orrù, Carla Esposito, Angela Amoresano, Raffaele Porta, Loredana Mariniello, Anna Cozzolino |
| المساهمون: | C., Esposito, Mariniello, Loredana, A., Cozzolino, Amoresano, Angela, S., Orru, Porta, Raffaele |
| المصدر: | Biochemistry. 40:4966-4971 |
| بيانات النشر: | American Chemical Society (ACS), 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Male, Spermidine, Tissue transglutaminase, Blotting, Western, Molecular Sequence Data, Kinesins, Biochemistry, Substrate Specificity, Mice, HSPA2, Animals, Secretion, Amino Acid Sequence, Carbon Radioisotopes, Rats, Wistar, Gene, chemistry.chemical_classification, Transglutaminases, Sequence Homology, Amino Acid, biology, Molecular Motor Proteins, Seminal Plasma Proteins, Proteins, Seminal Vesicles, In vitro, Rats, Amino acid, Enzyme, chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, biology.protein, Kinesin |
| الوصف: | By a proteomic approach, we demonstrated in rat coagulating gland secretion the presence of a 120 kDa protein which shares at least 80% identity at the amino acid level with the most closely related kinesin heavy chain codified by the kinesin superfamily protein Kif5c gene. In addition, we identified 30 and 66 kDa proteolytic fragments of such a kinesin heavy chain-like protein, corresponding to the 73- 299 N-terminal and 300-860 C-terminal regions, respectively. Finally, we demonstrated the occurrence in coagulating gland secretion of a 200 kDa protein probably derived by cross-linking reaction of the kinesin heavy chain-like protein with type IV transglutaminase. In fact, kinesin heavy chain-like protein and its 66 kDa proteolytic fragment were also found to act as effective acyl donor substrates for the enzyme in vitro. |
| وصف الملف: | STAMPA |
| تدمد: | 1520-4995 0006-2960 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22ddfdf4c45795da7ab98911514333fcTest https://doi.org/10.1021/bi001542wTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....22ddfdf4c45795da7ab98911514333fc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204995 00062960 |
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