Molecular Mapping of Tumor Heterogeneity on Clinical Tissue Specimens with Multiplexed Quantum Dots
| العنوان: | Molecular Mapping of Tumor Heterogeneity on Clinical Tissue Specimens with Multiplexed Quantum Dots |
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| المؤلفون: | Matthew L. Caldwell, Vijay Varma, Brian Leyland-Jones, Adeboye O. Osunkoya, Tracey Krogstad, Tao Liu, Jian Liu, May D. Wang, John A. Petros, Shuming Nie, Richard A. Moffitt, Stephen K. Lau, Andrew N. Young |
| المصدر: | ACS Nano. 4:2755-2765 |
| بيانات النشر: | American Chemical Society (ACS), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Immunoconjugates, medicine.medical_treatment, General Physics and Astronomy, Biology, Article, Mice, Cytokeratin, Prostate cancer, Prostate, Quantum Dots, Biomarkers, Tumor, medicine, Animals, Humans, Nanotechnology, General Materials Science, Staining and Labeling, Prostatectomy, General Engineering, Prostatic Neoplasms, Reproducibility of Results, medicine.disease, Molecular biology, Molecular Imaging, Spectrometry, Fluorescence, medicine.anatomical_structure, Quantum dot, Cancer cell, Cancer research, Immunohistochemistry, Rabbits, Molecular imaging |
| الوصف: | Tumor heterogeneity is one of the most important and challenging problems not only in studying the mechanisms of cancer development but also in developing therapeutics to eradicate cancer cells. Here we report the use of multiplexed quantum dots (QDs) and wavelength-resolved spectral imaging for molecular mapping of tumor heterogeneity on human prostate cancer tissue specimens. By using a panel of just four protein biomarkers (E-cadherin, high-molecular-weight cytokeratin, p63, and alpha-methylacyl CoA racemase), we show that structurally distinct prostate glands and single cancer cells can be detected and characterized within the complex microenvironments of radical prostatectomy and needle biopsy tissue specimens. The results reveal extensive tumor heterogeneity at the molecular, cellular, and architectural levels, allowing direct visualization of human prostate glands undergoing structural transitions from a double layer of basal and luminal cells to a single layer of malignant cells. For clinical diagnostic applications, multiplexed QD mapping provides correlated molecular and morphological information that is not available from traditional tissue staining and molecular profiling methods. |
| تدمد: | 1936-086X 1936-0851 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3756f8de3fb6140dc448fec114eef948Test https://doi.org/10.1021/nn100213vTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3756f8de3fb6140dc448fec114eef948 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1936086X 19360851 |
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