Blood Compatibility of Iron-Doped Nanosize Hydroxyapatite and Its Drug Release
| العنوان: | Blood Compatibility of Iron-Doped Nanosize Hydroxyapatite and Its Drug Release |
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| المؤلفون: | K. Elayaraja, Ganesh Venkatraman, R. Mythili, S. Narayana Kalkura, Ganga Baskar, W. Sofi Beaula, M.I. Ahymah Joshy, R.V. Suganthi, V. Sarath Chandra |
| المصدر: | ACS Applied Materials & Interfaces. 4:1200-1210 |
| بيانات النشر: | American Chemical Society (ACS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | inorganic chemicals, Materials science, Cell Survival, Iron, Analytical chemistry, Microbial Sensitivity Tests, Dielectric, Hemolysis, Cell Line, Magnetics, Crystallinity, Drug Delivery Systems, Electricity, X-Ray Diffraction, Hardness, Materials Testing, Spectroscopy, Fourier Transform Infrared, Humans, General Materials Science, Particle Size, Fourier transform infrared spectroscopy, Bacteria, Amoxicillin, Kinetics, Durapatite, Transmission electron microscopy, Vickers hardness test, X-ray crystallography, Nanoparticles, Crystallite, Inductively coupled plasma, Crystallization |
| الوصف: | Nanosize hydroxyapatite (nHAp) doped with varying levels of Fe(3+) (Fe-nHAp of average size 75 nm) was synthesized by hydrothermal and microwave techniques. The samples were characterized for physiochemical properties by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), inductively coupled plasma optical emission spectrometer (ICP-OES), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), mechanical and dielectric properties. The biological properties like hemocompatibility, antibacterial efficacy, in vitro bioactivity and the cell proliferation of the samples were determined. XRD pattern of the samples were of single phase hydroxyapatite. As the content of Fe(3+) increased, the crystallite size as well as crystallinity decreased along with a morphological change from spherulites to rods. The dielectric constants and Vickers hardness were enhanced on Fe(3+) doping. The VSM studies revealed that the saturation magnetization (M(s)) and retentivity (M(r)) were found to increase for Fe-nHAp. nHAp impregnated with an antibiotic as a new system for drug delivery in the treatment of chronic osteomyelitis was also attempted. The in vitro drug release with an antibiotic amoxicillin and anticancer drug 5-fluorouracil showed sustained release for the lowest concentration of Fe(3+), while with an increase in the content; there was a rapid release of the drug. The hemolytic assay of Fe(3+) doped samples revealed high blood compatibility (5% hemolysis). The antibacterial activities of the antibiotic impregnated materials were tested against a culture of E. coli, S. epidermidis and S. aureus by agar diffusion test. The in vitro bioactivity test using simulated body fluid (SBF) showed better bone bonding ability by the formation of an apatite layer on the doped samples. The growth of the apatite layer on the samples surface has been confirmed by EDS analysis. The proliferative potential of MG63 cells by MTT assay confirmed the noncytotoxicity of the samples. |
| تدمد: | 1944-8252 1944-8244 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98c5d53a7ec6313af01085431324aa6fTest https://doi.org/10.1021/am300140qTest |
| رقم الانضمام: | edsair.doi.dedup.....98c5d53a7ec6313af01085431324aa6f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19448252 19448244 |
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