Efficacy of IgG and F(ab′)2 Antivenoms to Neutralize Snake Venom-induced Local Tissue Damage as Assessed by the Proteomic Analysis of Wound Exudate
| العنوان: | Efficacy of IgG and F(ab′)2 Antivenoms to Neutralize Snake Venom-induced Local Tissue Damage as Assessed by the Proteomic Analysis of Wound Exudate |
|---|---|
| المؤلفون: | Jay W. Fox, Mauren Villalta, Teresa Escalante, John D. Shannon, Alexandra Rucavado, José María Gutiérrez, Carla N. Ayala-Castro |
| المصدر: | Journal of Proteome Research; Volumen 11, Número 1. 2012 Kérwá Universidad de Costa Rica instacron:UCR |
| بيانات النشر: | American Chemical Society (ACS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Proteomics, Snake venom, F(ab0)2, Proteome, IgG, Bothrops asper, Wound exudate, Hemorrhage, Biology, complex mixtures, Myonecrosis, Biochemistry, Immunoglobulin Fab Fragments, Mice, Antivenom, Crotalid Venoms, Tissue damage, Animals, Bothrops, Horses, Muscle, Skeletal, Extracellular Matrix Proteins, Antivenins, Wound.exudate, Blood Proteins, Exudates and Transudates, Extracellular matrix, General Chemistry, Immunoglobulin G, Immunology |
| الوصف: | 2082-01 Embargo por política editorial Proteomic analysis of wound exudates represents a valuable tool to investigate tissue pathology and to assess the therapeutic success of various interventions. In this study, the ability of horse-derived IgG and F(ab0)2 antivenoms to neutralize local pathological effects induced by the venom of the snake Bothrops asper in mouse muscle was investigated by the proteomic analysis of exudates collected in the vicinity of affected tissue. In experiments involving the incubation of venom and antivenom prior to injection in mice, hemorrhagic activity was completely abolished and local muscle damaging activity was significantly reduced by the antivenoms. In these conditions, the relative amounts of several intracellular and extracellular matrix proteins were reduced by the action of antivenoms, whereas the relative amounts of various plasma proteins were not modified. Because not all intracellular proteins were reduced, it is likely that there is a residual cytotoxicity not neutralized by antivenoms. In experiments designed to more closely reproduce the actual circumstances of envenoming, that is, when antivenom is administered after envenomation, the number of proteins whose amounts in exudates were reduced by antivenoms decreased, underscoring the difficulty in neutralizing local pathology due to the very rapid onset of venom-induced pathology. In these experiments, IgG antivenom was more efficient than F(ab0)2 antivenom when administered after envenomation, probably as a consequence of differences in their pharmacokinetic profiles. Universidad de Costa Rica/[741-A9-003]/UCR/Costa Rica Universidad de Costa Rica/[741-B0-528]/UCR/Costa Rica Network for Research and Training in Tropical Diseases in Central America /[01-N-2010]/NeTropica/ UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) |
| تدمد: | 1535-3907 1535-3893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0ab7f5cb729fdcd133a927e941a9992Test https://doi.org/10.1021/pr200847qTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f0ab7f5cb729fdcd133a927e941a9992 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
|---|