Molecular cloning and tissue distribution of alternatively spliced mRNAs encoding possible mammalian homologs of the yeast secretory pathway calcium pump
| العنوان: | Molecular cloning and tissue distribution of alternatively spliced mRNAs encoding possible mammalian homologs of the yeast secretory pathway calcium pump |
|---|---|
| المؤلفون: | Gary E. Shull, David M. Clarke, Ann Marie Gunteski-Hamblin |
| المصدر: | Biochemistry. 31:7600-7608 |
| بيانات النشر: | American Chemical Society (ACS), 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Male, Protein Conformation, RNA Splicing, Molecular Sequence Data, Restriction Mapping, Calcium-Transporting ATPases, Saccharomyces cerevisiae, Molecular cloning, Biology, Kidney, Biochemistry, Homology (biology), Restriction map, Sequence Homology, Nucleic Acid, Complementary DNA, Testis, Animals, Coding region, Amino Acid Sequence, RNA, Messenger, Northern blot, Cloning, Molecular, Gene Library, Base Sequence, Stomach, Nucleic acid sequence, Brain, DNA, Blotting, Northern, Molecular biology, Rats, Sarcoplasmic Reticulum, Organ Specificity, RNA, Poly A, Oligomer restriction |
| الوصف: | Rat stomach and testis cDNAs corresponding to two alternatively spliced mRNAs encoding variants of a P-type ion-transport ATPase that closely resembles the yeast secretory pathway Ca2+ pump have been isolated and characterized. A partial kidney cDNA was identified previously using an oligonucleotide probe corresponding to part of the sarcoplasmic reticulum Ca(2+)-ATPase [Gunteski-Hamblin, A., Greeb, J., & Shull, G.E. (1988) J. Biol. Chem. 263, 15032-15040]. In the present study, we first isolated and characterized a stomach cDNA that contains the entire coding sequence. The 919 amino acid enzyme has the same apparent transmembrane organization and contains all of the conserved domains present in other P-type ATPases. Northern blot analyses demonstrate that 3.9- and 5-kilobase mRNAs corresponding to the cDNA were present in all tissues examined, suggesting that the protein it encodes performs a housekeeping function. Rat testis also contained a 3.7-kilobase mRNA that hybridized with a probe from the 5' end of the stomach cDNA but did not hybridize with a probe from the 3' end. Cloning and characterization of cDNAs corresponding to the smaller testis mRNA revealed that it is derived from the same gene but encodes a variant of the enzyme in which the C-terminal residue, Val-919, is replaced by the sequence Phe-919-Tyr-Pro-Lys-Ile-923. Similarity comparisons show that the two enzymes are more closely related to the known Ca2+ pumps than to other P-type ATPases.(ABSTRACT TRUNCATED AT 250 WORDS) |
| تدمد: | 1520-4995 0006-2960 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::795031c57e0facf4a0a3e8151d867e79Test https://doi.org/10.1021/bi00148a023Test |
| رقم الانضمام: | edsair.doi.dedup.....795031c57e0facf4a0a3e8151d867e79 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204995 00062960 |
|---|