Detection of Carbofuran-Protein Adducts in Serum of Occupationally Exposed Pesticide Factory Workers in Pakistan
| العنوان: | Detection of Carbofuran-Protein Adducts in Serum of Occupationally Exposed Pesticide Factory Workers in Pakistan |
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| المؤلفون: | Muhammad Aslam Shad, Young Ah Goo, Tanzila Rehman, Mazhar Hussain, David R. Goodlett, Mohd M. Khan, Benjamin L. Oyler |
| المصدر: | Chemical Research in Toxicology. 29:1720-1728 |
| بيانات النشر: | American Chemical Society (ACS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Chromatography, biology, Arginine, Serum albumin, General Medicine, Toxicology, Blood proteins, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, Biochemistry, chemistry, Liquid chromatography–mass spectrometry, 030220 oncology & carcinogenesis, Lactate dehydrogenase, biology.protein, Alkaline phosphatase, Creatine kinase, Carbofuran |
| الوصف: | This study was conducted to investigate the protein adducts with pesticides in a cohort of 172 factory workers that were exposed to a mixture of pesticides. The 35 samples showing considerable variation in biochemical parameters, i.e., butyrylcholinestrase (BChE), serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), gamma-glutamyl transferase (GGT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP/ALKP), lactate dehydrogenase (LDH), creatine phosphokinase (CPK) enzymes, and controls were analyzed by reversed-phase nanoscale liquid chromatography tandem mass spectrometry (nLC-MS/MS) on an Orbitrap mass spectrometer employing a shotgun proteomics approach. Only protein adducts with carbofuran were found on serum proteins of these workers. These adducts were of carbofuran labeled lysine (Lys-142, Lys-183, Lys-287, and Lys-467), arginine (Arg-210, Arg-242, and Arg-256) from serum albumin, and serine (Ser-07, Ser-54, and Ser-150) from immunoglobulin proteins. The arginine residues (Arg-210, Arg-242, Arg-246, and Arg-434) from albumin were also found to be glycated in serum of workers showing a high level of glucose who also had glycated arginine (Arg-1120) modified with carbofuran in their tankyrase-1-binding protein. The number of tandem mass spectra of modified peptides increased with increasing time of exposure. This is the first report to demonstrate the presence of carbofuran-labeled albumin, immunoglobulin, and glycated arginine, which shows that lysine and arginine of human albumin and serine of immunoglobulin are covalently modified in the serum of workers that were occupationally exposed to carbofuran, and the modification is detectable by tandem mass spectrometry. These peptides modified with carbofuran can potentially be used as a biomarker of carbofuran exposure. |
| تدمد: | 1520-5010 0893-228X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7461f906daeff675b06987abc5fcc8f5Test https://doi.org/10.1021/acs.chemrestox.6b00222Test |
| رقم الانضمام: | edsair.doi.dedup.....7461f906daeff675b06987abc5fcc8f5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205010 0893228X |
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