Characterization of an antifungal and cryoprotective class i chitinase from table grape berries (Vitis vinifera Cv. Cardinal)
| العنوان: | Characterization of an antifungal and cryoprotective class i chitinase from table grape berries (Vitis vinifera Cv. Cardinal) |
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| المؤلفون: | M. Isabel Escribano, Oscar Goñi, Irene Romero, Carmen Merodio, Carlos Fernandez-Caballero, M. Teresa Sanchez-Ballesta |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | American Chemical Society, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Antifungal, medicine.drug_class, Molecular Sequence Data, Class I chitinase, Gene Expression, Berry, Biology, Cryoprotective Agents, Freezing, Gene expression, Botany, Escherichia coli, medicine, Vitis, Amino Acid Sequence, Vitis vinifera, Plant Proteins, L-Lactate Dehydrogenase, Chitinases, Table grape, General Chemistry, Recombinant Proteins, Fungicides, Industrial, Fruit, Chitinase, biology.protein, sense organs, General Agricultural and Biological Sciences, Sequence Alignment |
| الوصف: | Gene expression of a class I chitinase (Vcchit1b) in the skin of table grapes was analyzed as a molecular marker for changes induced at low temperature and also to study the effect of high CO2 levels modulating transcript levels at 0 °C. An active recombinant VcCHIT1b was overexpressed in Escherichia coli, and as the protein was produced as insoluble inclusion bodies, it was solubilized and refolded. The purified recombinant chitinase showed an optimum pH of 6.0 and a temperature of 50 °C, retaining activity at 0 and -10 °C. Purified chitinase exerted in vitro antifungal activity against Botrytis cinerea. Furthermore, recombinant chitinase was able to cryoprotect lactate dehydrogenase against freeze/thaw inactivation. However, the recombinant VcCHIT1b did not show any antifreeze activity when the thermal hysteresis activity was measured using differential scanning calorimetry. © 2009 American Chemical Society. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::463463300d239747c50b8be011456292Test http://hdl.handle.net/10261/77736Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....463463300d239747c50b8be011456292 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |