Conformational Change and Activity Enhancement of Rabbit Muscle Lactate Dehydrogenase Induced by Polyethyleneimine
| العنوان: | Conformational Change and Activity Enhancement of Rabbit Muscle Lactate Dehydrogenase Induced by Polyethyleneimine |
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| المؤلفون: | Zilong Ren, Lin Ma, Min Zhang, Xiafan Xu, Chunlan Du |
| المصدر: | ACS Omega ACS Omega, Vol 6, Iss 16, Pp 10859-10865 (2021) |
| بيانات النشر: | American Chemical Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Conformational change, Circular dichroism, biology, Chemistry, General Chemical Engineering, technology, industry, and agriculture, Substrate (chemistry), General Chemistry, macromolecular substances, Cofactor, Article, Hydrophobic effect, chemistry.chemical_compound, Enzyme, Sodium pyruvate, Lactate dehydrogenase, biology.protein, Biophysics, QD1-999 |
| الوصف: | For a better understanding on the interaction between polyethyleneimine (PEI) and proteins, spectroscopic studies including UV-vis absorption, resonance Rayleigh scattering, fluorescence, and circular dichroism were conducted to reveal the conformational change of rabbit muscle lactate dehydrogenase (rmLDH) and related to the bioactivity of the enzyme. Regardless of the electrostatic repulsion, PEI could bind on the surface of rmLDH, a basic protein, via hydrogen binding of the dense amine groups and hydrophobic interaction of methyl groups. The competitive binding by PEI led to a reduction of the binding efficiency of rmLDH toward β-nicotinamide adenine dinucleotide, the coenzyme, and sodium pyruvate, the substrate. However, the complex formation with PEI induced a less ordered conformation and an enhanced surface hydrophobicity of rmLDH, facilitating the turnover of the enzyme and generally resulting in an increased activity. PEI of higher molecular weight was more efficient to induce alteration in the conformation and catalytic activity of the enzyme. |
| اللغة: | English |
| تدمد: | 2470-1343 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce97445409c87cbd05630bb1c6db950bTest http://europepmc.org/articles/PMC8153759Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ce97445409c87cbd05630bb1c6db950b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24701343 |
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