التفاصيل البيبلوغرافية
| العنوان: |
Tailoring Enzyme-Rich Environmental DNA Clones: A Source of Enzymes for Generating Libraries of Unnatural Natural Products. |
| المؤلفون: |
Banik, Jacob J.1, Craig, Jeffrey W.1, Calle, Paula Y.1, Brady, Sean F.1 sbrady@rockefeller.edu |
| المصدر: |
Journal of the American Chemical Society. 11/10/2010, Vol. 132 Issue 44, p15661-15670. 10p. |
| مصطلحات موضوعية: |
*BIOINFORMATICS, *GLYCOPEPTIDES, *ENZYME analysis, *SULFOTRANSFERASES, *ACTINOMYCETALES |
| مستخلص: |
A detailed bioinformatics analysis of six glycopeptide biosynthetic gene clusters isolated from soil environmental DNA (eDNA) megalibraries indicates that a subset of these gene clusters contains collections of tailoring enzymes that are predicted to result in the production of new glycopeptide congeners. In particular, sulfotransferases appear in eDNA-derived gene clusters at a much higher frequency than would be predicted from the characterization of glycopeptides from cultured Actinomycetes. Enzymes found on tailoring-enzyme-rich eDNA clones associated with these six gene clusters were used to produce a series of new sulfated glycopeptide derivatives in both in vitro and in vivo derivatization studies. The derivatization of known natural products with eDNA-derived tailoring enzymes is likely to be a broadly applicable strategy for generating libraries of new natural product variants. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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