التفاصيل البيبلوغرافية
| العنوان: |
Cross-Talk between Shpl and PIPKIγ Controls Leukocyte Recruitment. |
| المؤلفون: |
Stadtmann, Anika1,2, Block, Helena1,2, Volmering, Stephanie1,2, Abram, Clare3, Sohlbach, Charlotte1,2, Boras, Mark1,2, Lowell, Clifford A.3, Zarbock, Alexander1,2 zarbock@uni-muenster.de |
| المصدر: |
Journal of Immunology. 8/1/2015, Vol. 195 Issue 3, p1152-1161. 10p. |
| مصطلحات موضوعية: |
*PHOSPHATIDYLINOSITOL 3-kinases, *NEUTROPHILS, *CELL adhesion, *CHEMOTAXIS, *BIOLOGICAL crosstalk, *LEUCOCYTES |
| مستخلص: |
Neutrophil recruitment to the site of inflammation plays a pivotal role in host defense. However, overwhelming activation and accumulation of neutrophils in the tissue may cause tissue damage and autoimmunity due to the release of cytokines, oxidants, and proteases. Neutrophil adhesion in acute inflammation is initiated by activation of αLß2 (LFA-1), which can be induced by rolling on E-selectin (slowly) or by exposure to the chemokine CXCL1 (rapidly). Despite the clinical importance, cell-intrinsic molecular mechanisms of negative regulation of integrin adhesiveness and neutrophil recruitment are poorly understood. Mice deficient in the tyrosine phosphatase Src homology 2 domain-containing protein tyrosine phosphatase 1 (Shp1) show increased leukocyte adhesion, but the interpretation of these data is limited by the severe global phenotype of these mice. In this study, we used mice with global and myeloid-restricted deletion of Shp1 to study neutrophil arrest, adhesion, crawling, and transendothelial migration in vitro and in vivo. Shp1 deficiency results in increased neutrophil adhesion in vivo; however, neutrophil crawling, transmigration, and chemotaxis were reduced in these mice. Mechanistically, Shp1 binds and controls PIPKIγ activity and, thereby, modulates phosphatidylinositol (4,5)-bisphosphate levels and adhesion. Thus, Shp1 is involved in the deactivation of integrins and regulation of neutrophil recruitment into inflamed tissue. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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