التفاصيل البيبلوغرافية
| العنوان: |
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes |
| المؤلفون: |
Harrington, Lucas B., Burstein, David, Chen, Janice S., Paez-Espino, David, Ma, Enbo, Witte, Isaac P., Cofsky, Joshua C., Kyrpides, Nikos C., Banfield, Jillian F., Doudna, Jennifer A. |
| المساهمون: |
National Science Foundation, U.S. Department of Energy |
| المصدر: |
Science ; volume 362, issue 6416, page 839-842 ; ISSN 0036-8075 1095-9203 |
| بيانات النشر: |
American Association for the Advancement of Science (AAAS) |
| سنة النشر: |
2018 |
| الوصف: |
A programmable type of CRISPR system CRISPR-Cas9 systems have been causing a revolution in biology. Harrington et al. describe the discovery and technological implementation of an additional type of CRISPR system based on an extracompact effector protein, Cas14. Metagenomics data, particularly from uncultivated samples, uncovered the CRISPR-Cas14 systems containing all the components necessary for adaptive immunity in prokaryotes. At half the size of class 2 CRISPR effectors, Cas14 appears to target single-stranded DNA without class 2 sequence restrictions. By leveraging this activity, a fast and high-fidelity nucleic acid detection system enabled detection of single-nucleotide polymorphisms. Science , this issue p. 839 |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1126/science.aav4294 |
| الإتاحة: |
https://doi.org/10.1126/science.aav4294Test |
| رقم الانضمام: |
edsbas.3534EEF3 |
| قاعدة البيانات: |
BASE |
