Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca 2+ and cAMP Signaling
| العنوان: | Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca 2+ and cAMP Signaling |
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| المؤلفون: | Michelle L. Halls, Jonathan Pacheco, Katy L. Everett, Debbie Willoughby, Philipp Skroblin, Enno Klussmann, Luis Vaca, Dermot M.F. Cooper |
| المصدر: | Science Signaling. 5 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | ORAI1 Protein, chemistry.chemical_element, Calcium, Biology, Biochemistry, chemistry.chemical_compound, Bacterial Proteins, Cyclic AMP, Fluorescence Resonance Energy Transfer, Homeostasis, Humans, Cyclic adenosine monophosphate, Molecular Biology, Ions, SOC channels, ORAI1, Colocalization, Cell Biology, Protein Structure, Tertiary, Cell biology, Luminescent Proteins, Crosstalk (biology), HEK293 Cells, chemistry, Calcium Channels, Signal transduction, Adenylyl Cyclases |
| الوصف: | The interplay between calcium ion (Ca(2+)) and cyclic adenosine monophosphate (cAMP) signaling underlies crucial aspects of cell homeostasis. The membrane-bound Ca(2+)-regulated adenylyl cyclases (ACs) are pivotal points of this integration. These enzymes display high selectivity for Ca(2+) entry arising from the activation of store-operated Ca(2+) (SOC) channels, and they have been proposed to functionally colocalize with SOC channels to reinforce crosstalk between the two signaling pathways. Using a multidisciplinary approach, we have identified a direct interaction between the amino termini of Ca(2+)-stimulated AC8 and Orai1, the pore component of SOC channels. High-resolution biosensors targeted to the AC8 and Orai1 microdomains revealed that this protein-protein interaction is responsible for coordinating subcellular changes in both Ca(2+) and cAMP. The demonstration that Orai1 functions as an integral component of a highly organized signaling complex to coordinate Ca(2+) and cAMP signals underscores how SOC channels can be recruited to maximize the efficiency of the interplay between these two ubiquitous signaling pathways. |
| تدمد: | 1937-9145 1945-0877 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfa7350f062d106e003dc498da698896Test https://doi.org/10.1126/scisignal.2002299Test |
| رقم الانضمام: | edsair.doi.dedup.....cfa7350f062d106e003dc498da698896 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19379145 19450877 |
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