Menin 'reads' H3K79me2 mark in a nucleosomal context
| العنوان: | Menin 'reads' H3K79me2 mark in a nucleosomal context |
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| المؤلفون: | Jianwei Lin, Yiping Wu, Gaofei Tian, Daqi Yu, Eunjeong Yang, Wai Hei Lam, Zheng Liu, Yihang Jing, Shangyu Dang, Xiucong Bao, Jason Wing Hon Wong, Yuanliang Zhai, Xiang David Li |
| المصدر: | Science. 379:717-723 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | Methylation of histone H3 lysine-79 (H3K79) is an epigenetic mark for gene regulation in development, cellular differentiation, and disease progression. However, how this histone mark is translated into downstream effects remains poorly understood owing to a lack of knowledge about its readers. We developed a nucleosome-based photoaffinity probe to capture proteins that recognize H3K79 dimethylation (H3K79me2) in a nucleosomal context. In combination with a quantitative proteomics approach, this probe identified menin as a H3K79me2 reader. A cryo–electron microscopy structure of menin bound to an H3K79me2 nucleosome revealed that menin engages with the nucleosome using its fingers and palm domains and recognizes the methylation mark through a π-cation interaction. In cells, menin is selectively associated with H3K79me2 on chromatin, particularly in gene bodies. |
| تدمد: | 1095-9203 0036-8075 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e8dd1919c1d597ecfb5780fa24209363Test https://doi.org/10.1126/science.adc9318Test |
| رقم الانضمام: | edsair.doi...........e8dd1919c1d597ecfb5780fa24209363 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959203 00368075 |
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