Phosphorylation of the Autophagy Receptor Optineurin Restricts Salmonella Growth
| العنوان: | Phosphorylation of the Autophagy Receptor Optineurin Restricts Salmonella Growth |
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| المؤلفون: | Sebastian A. Wagner, Oliver Waidmann, Benjamin Richter, Volker Dötsch, Philipp Wild, Chunaram Choudhary, Jelena Korac, Dirk Bumann, Nathan R. Brady, Vladimir V. Rogov, Hesso Farhan, David G. McEwan, Ivan Dikic |
| المصدر: | Science Europe PubMed Central |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Salmonella typhimurium, Sequestosome-1 Protein, optineurin, autophagy, salmonella, TBK1, phosphorylation, Cell Cycle Proteins, Aggrephagy, Protein Serine-Threonine Kinases, Biology, BAG3, Models, Biological, Article, 03 medical and health sciences, Cytosol, 0302 clinical medicine, Cell Line, Tumor, Transcription Factor TFIIIA, Autophagy, Xenophagy, Humans, Protein Interaction Domains and Motifs, Phosphorylation, Protein kinase A, Adaptor Proteins, Signal Transducing, 030304 developmental biology, Optineurin, 0303 health sciences, Multidisciplinary, Ubiquitin, Membrane Transport Proteins, Nuclear Proteins, Immunity, Innate, 3. Good health, Cell biology, Biochemistry, RNA Interference, Microtubule-Associated Proteins, 030217 neurology & neurosurgery, HeLa Cells, Protein Binding |
| الوصف: | Selective autophagy can be mediated via receptor molecules that link specific cargoes to the autophagosomal membranes decorated by ubiquitin-like microtubule-associated protein light chain 3 (LC3) modifiers. Although several autophagy receptors have been identified, little is known about mechanisms controlling their functions in vivo. In this work, we found that phosphorylation of an autophagy receptor, optineurin, promoted selective autophagy of ubiquitin-coated cytosolic Salmonella enterica. The protein kinase TANK binding kinase 1 (TBK1) phosphorylated optineurin on serine-177, enhancing LC3 binding affinity and autophagic clearance of cytosolic Salmonella. Conversely, ubiquitin- or LC3-binding optineurin mutants and silencing of optineurin or TBK1 impaired Salmonella autophagy, resulting in increased intracellular bacterial proliferation. We propose that phosphorylation of autophagy receptors might be a general mechanism for regulation of cargo-selective autophagy. |
| تدمد: | 1095-9203 0036-8075 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30ed66d866c8d28aa152f85ff2e1b37aTest https://doi.org/10.1126/science.1205405Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....30ed66d866c8d28aa152f85ff2e1b37a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959203 00368075 |
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