دورية أكاديمية
Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2
| العنوان: | Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2 |
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| المؤلفون: | Yoshiba, Satoko, Shiratori, Hidetaka, Kuo, Ivana Y., Kawasumi, Aiko, Shinohara, Kyosuke, Nonaka, Shigenori, Asai, Yasuko, Sasaki, Genta, Belo, José A., Sasaki, Hiroshi, Nakai, Junichi, Dworniczak, Bernd, Ehrlich, Barbara E., Pennekamp, Petra, Hamada, Hiroshi |
| بيانات النشر: | American Association for the Advancement of Science |
| سنة النشر: | 2012 |
| المجموعة: | Universidade do Algarve: Sapienta |
| مصطلحات موضوعية: | Polycystic kidney disease, Left-right asymmetry, Left-right axis, Mice, Channel, Protein, Expression, Release, Lacking, Kif3A |
| الوصف: | Unidirectional fluid flow plays an essential role in the breaking of left-right (L-R) symmetry in mouse embryos, but it has remained unclear how the flow is sensed by the embryo. We report that the Ca2+ channel Polycystin-2 (Pkd2) is required specifically in the perinodal crown cells for sensing the nodal flow. Examination of mutant forms of Pkd2 shows that the ciliary localization of Pkd2 is essential for correct L-R patterning. Whereas Kif3a mutant embryos, which lack all cilia, failed to respond to an artificial flow, restoration of primary cilia in crown cells rescued the response to the flow. Our results thus suggest that nodal flow is sensed in a manner dependent on Pkd2 by the cilia of crown cells located at the edge of the node. ; CREST of the Japan Science and Technology Corporation; NIH [P30 DK090744]; Human Frontier Science Program [ST00246/2003C]; Deutsche Forschungsgemeinschaft [PE 853/2]; Japan Society for the Promotion of Science; American Heart Association [R10682] ; info:eu-repo/semantics/publishedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0036-8075 |
| العلاقة: | http://hdl.handle.net/10400.1/11866Test; https://doi.org/10.1126/science.1222538Test |
| DOI: | 10.1126/science.1222538 |
| الإتاحة: | https://doi.org/10.1126/science.1222538Test http://hdl.handle.net/10400.1/11866Test |
| حقوق: | openAccess ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.84AE2FC9 |
| قاعدة البيانات: | BASE |
| تدمد: | 00368075 |
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| DOI: | 10.1126/science.1222538 |