Regulation of Cyclin D1 RNA Stability by SNIP1
| العنوان: | Regulation of Cyclin D1 RNA Stability by SNIP1 |
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| المؤلفون: | Neil D. Perkins, Steven J. Wall, Benjamin Barré, Paul Ajuh, Kostya I. Panov, Cameron P. Bracken |
| المساهمون: | Bracken, Cameron P, Wall, Steven J, Barre, Benjamin, Panov, Kostya I, Ajuh, Paul M, Perkins, Neil D |
| المصدر: | Cancer Research. 68:7621-7628 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Cancer Research, Cyclin E, Transcription, Genetic, Cyclin D, Cyclin A, cyclin D1, Cyclin B, Bone Neoplasms, Transfection, Polymerase Chain Reaction, Article, Cyclin D1, Cyclin-dependent kinase, Cell Line, Tumor, Humans, RNA, Messenger, RNA, Neoplasm, RNA, Small Interfering, Osteosarcoma, biology, messenger RNA, Cell Cycle, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, RNA-Binding Proteins, nucleocytoplasmic transport protein, Splicing Factor U2AF, Molecular biology, Cell biology, Gene Expression Regulation, Neoplastic, Ribonucleoproteins, Oncology, Cyclin-dependent kinase complex, biology.protein, bclaf1 protein, Cyclin A2, HeLa Cells |
| الوصف: | Cyclin D1 expression represents one of the key mitogen-regulated events during the G1 phase of the cell cycle, whereas Cyclin D1 overexpression is frequently associated with human malignancy. Here, we describe a novel mechanism regulating Cyclin D1 levels. We find that SNIP1, previously identified as a regulator of Cyclin D1 expression, does not, as previously thought, primarily function as a transcriptional coactivator for this gene. Rather, SNIP1 plays a critical role in cotranscriptional or posttranscriptional Cyclin D1 mRNA stability. Moreover, we show that the majority of nucleoplasmic SNIP1 is present within a previously undescribed complex containing SkIP, THRAP3, BCLAF1, and Pinin, all proteins with reported roles in RNA processing and transcriptional regulation. We find that this complex, which we have termed the SNIP1/SkIP–associated RNA-processing complex, is coordinately recruited to both the 3′ end of the Cyclin D1 gene and Cyclin D1 RNA. Significantly, SNIP1 is required for the further recruitment of the RNA processing factor U2AF65 to both the Cyclin D1 gene and RNA. This study shows a novel mechanism regulating Cyclin D1 expression and offers new insight into the role of SNIP1 and associated proteins as regulators of proliferation and cancer. [Cancer Res 2008;68(18):7621–8] |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fd0c029bb556097b98a5aa3b3496e68Test https://doi.org/10.1158/0008-5472.can-08-1217Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0fd0c029bb556097b98a5aa3b3496e68 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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