The Generation and Regulation of Functional Diversity of Malignant Plasma Cells
| العنوان: | The Generation and Regulation of Functional Diversity of Malignant Plasma Cells |
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| المؤلفون: | Liat Nadav, Nir Cohen, Benjamin Geiger, Shoshana Baron, Ben-Zion Katz, Elizabeth Naparstek |
| المصدر: | Cancer Research. 66:8608-8616 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Integrins, Cancer Research, Pathology, medicine.medical_specialty, Plasma Cells, Population, Biology, Plasma cell, Cell Line, Flow cytometry, Trinucleotide Repeats, Cell Line, Tumor, Cell Adhesion, medicine, Humans, education, Multiple myeloma, education.field_of_study, Leukemia, CD40, medicine.diagnostic_test, Genetic Variation, medicine.disease, Molecular biology, Cell Transformation, Neoplastic, medicine.anatomical_structure, Oncology, Cell culture, biology.protein, Bone marrow, Paxillin, Multiple Myeloma, Homing (hematopoietic) |
| الوصف: | Cellular diversity, which is a hallmark of malignancy, can be generated by both genetic and nongenetic mechanisms. We describe here variability in the adhesive and migratory behavior of malignant plasma cell populations, including multiple myeloma–derived lines and primary patient samples. Examination of the plasma cell lines ARH-77, CAG, and AKR revealed two distinct subpopulations of cells, one displaying highly adhesive properties (type A) and the other consisting of poorly adhesive, floating cells (type F). In the ARH-77 cell line, type A cells attach better to fibronectin and to human bone fragments and form paxillin-rich focal adhesions, whereas type F cells are highly motile and exert integrin-dependent bone marrow homing capacity in nonobese diabetic/severe combined immunodeficient mice. Flow cytometry indicated that type A cells express significantly higher levels of CD45 and CD56 and lower levels of CD138 compared with type F cells. Interestingly, culturing of either type A or type F cells under nonselective conditions resulted in the development of mixed cell population similar to the parental ARH-77 cells. Analysis of bone marrow aspirates of multiple myeloma patients revealed that spicules within the aspirates are enriched with type A–like cells. Nonadherent cells within the aspirate fluids express a marker profile similar to type F cells. This study indicates that multiple myeloma patients contain heterogeneous populations of malignant plasma cells that display distinct properties. Diverse subpopulations of malignant plasma cells may play distinct roles in the different biological and clinical manifestations of plasma cell dyscrasias, including bone dissemination and selective adhesion to bone marrow compartments. (Cancer Res 2006; 66(17): 8608-16) |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::433f534e62960235cfea4ca07b796657Test https://doi.org/10.1158/0008-5472.can-06-1301Test |
| رقم الانضمام: | edsair.doi.dedup.....433f534e62960235cfea4ca07b796657 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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