Abstract 824: Comparison of the high risk HPV detection methods (HC II, DNA Chip, and luminex-based assay) in cervical lesion of Korean women
| العنوان: | Abstract 824: Comparison of the high risk HPV detection methods (HC II, DNA Chip, and luminex-based assay) in cervical lesion of Korean women |
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| المؤلفون: | Su-Mi Bae, Dong Chun Park, Ku Hee Kim, Yong Wook Kim, Yun Sun Kim, Jae Eun Shin, Woong Shick Ahn |
| المصدر: | Cancer Research. 70:824-824 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cervical cancer, Cancer Research, HPV infection, Cancer, Biology, medicine.disease, Virology, DNA sequencing, law.invention, chemistry.chemical_compound, Oncology, chemistry, High risk hpv, law, medicine, DNA microarray, Polymerase chain reaction, DNA |
| الوصف: | The detection of human papillomavirus (HPV) of female genital tract is very important, several HPV DNA detection methods have been developed during the last decade. In previous report, we introduced luminex-based HPV genotyping assay that is combined PCR amplification. In this study, we compared performances to detect high risk HPV types in hybrid capture 2 (HC II), DNA Chip, and luminex-based assay. A total of 330 cervical swabs from Cervical Cancer Early Diagnostic Clinic, Seoul St. Mary's Hospital were investigated. DNA was extracted from a single sample and HPV DNA detection assays were performed on the same specimen with 3 strategies and confirmed by DNA sequencing. Among 330 cervical swabs, 230 were HPV positive, 100 were negative. HPV detection rates of Luminex, HC II and DNA Chip in 330 specimens were 65.2%, 61.2% and 68.8%, respectively. The results of DNA sequence analysis, accuracy of Luminex, HC II and DNA Chip was 91.2%, 82.7% and 80.9%, respectively. HPV DNA infection slightly increase with severity of disease in cytologic and histologic diagnosis, there is no correlation between HPV, because HPV DNAs were detected in many samples with normal in this study. Conclusively in HPV detection comparison, DNA Chip had relatively high detection rate than Luminex and HC II, but Luminex had the highest accuracy for HPV DNA detection. These results indicate that the Luminex-based assay has more accurate performance than DNA Chip and HC II assay in the detection of HPV infections. We suggest that Luminex-based assay may be useful method for detecting of HPV infection clinically. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 824. |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::b25cd3cf47b3a937e8142a41b093455bTest https://doi.org/10.1158/1538-7445.am10-824Test |
| رقم الانضمام: | edsair.doi...........b25cd3cf47b3a937e8142a41b093455b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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