Abstract 2870: Serine/arginine rich proteins show different distribution patterns in stratum of oral epidermoid carcinoma
| العنوان: | Abstract 2870: Serine/arginine rich proteins show different distribution patterns in stratum of oral epidermoid carcinoma |
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| المؤلفون: | Javier Hernandez, Luz Ruíz Godoy Rivera, Erika Ruiz-García, Reyna Lara-Martínez, Lourdes Segura-Valdés, Abelardo Meneses-García, Cynthia J. Ceja-Lopez, Luis Felipe Jiménez García, Jorge Alberto Guadarrama-Orozco |
| المصدر: | Cancer Research. 76:2870-2870 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Cancer Research, SR protein, Oncology, Biochemistry, Epidermoid carcinoma, Cytoplasm, Alternative splicing, RNA splicing, Gene expression, Biology, Interchromatin granule, Molecular biology, Immunostaining |
| الوصف: | Serine/arginine proteins increased their activity during the alternative splicing in cell nucleus. The nuclear speckles, also known as interchromatin granule clusters, are enriched with SR splicing factors and are implicated in gene expression. Nuclear speckle formation is developmentally regulated; in certain cases phosphorylated SR proteins are absent from the nucleus and are instead localized at granular structures in the cytoplasm. SR proteins are related with splicing during different types of cancers and metástasis. We search for phosphorylated SR proteins and the nuclear pattern and speckle formation in human oral carcinomas. Methods: To address nuclear pattern and speckle formation, we performed a series of immunostaining experiments, with SC35 antibody for phosphorylated srsf2 on sections derived from oral epidermoid tumor from 33 patients. The images were obtained with a microscope (Leica TCS SPE/CTR 4000). For imaging cytometry analyses, a Leica LAS AF Lite software (Leica Microsystems) was used to capture images automatically; images and recognition of nuclear speckles were quantitatively analyzed using analysis software (ImageJ). ANOVA analysis was performed for the cellular and staining correlation. Results: There are significant differences in morphology and distribution of intranuclear mottled pattern of SR proteins in each tissue layer between oral epithelial without neoplasia and oral squamous cell carcinoma (P < 0.0001). We also found that number, size and pattern (p We found that is in the granulosum stratum of oral squamous carcinomas where the SR proteins shows more transcriptional activity. This finding could be related with the increased metabolism of this layer. Citation Format: Cynthia J. Ceja-Lopez, Reyna Lara-Martínez, Lourdes Segura-Valdés, Luz Ruíz Godoy Rivera, Javier A. Ambrosio Hernandez, Jorge Alberto Guadarrama-Orozco, Erika Ruíz-García, Luis Felipe Jiménez García, Abelardo Meneses-García. Serine/arginine rich proteins show different distribution patterns in stratum of oral epidermoid carcinoma. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 2870. |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::8fbc25a3eabc36fe340c3ed03158af7bTest https://doi.org/10.1158/1538-7445.am2016-2870Test |
| رقم الانضمام: | edsair.doi...........8fbc25a3eabc36fe340c3ed03158af7b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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