Identification of Peptide Vaccine Candidates for Prostate Cancer Patients with HLA-A3 Supertype Alleles
| العنوان: | Identification of Peptide Vaccine Candidates for Prostate Cancer Patients with HLA-A3 Supertype Alleles |
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| المؤلفون: | Mamoru Harada, Kyogo Itoh, Masanori Noguchi, Satoko Matsueda, Hiroko Takedatsu, Akihisa Yao, Masahiro Tanaka |
| المصدر: | Clinical Cancer Research. 11:6933-6943 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Glutamate Carboxypeptidase II, Male, Cancer Research, Acid Phosphatase, Amino Acid Motifs, Epitopes, T-Lymphocyte, HLA-A3 Antigen, Cancer Vaccines, Immunoglobulin G, HLA-A11 Antigen, Prostate cancer, Antigen, Prostate, Cell Line, Tumor, medicine, Humans, Alleles, HLA-A Antigens, biology, business.industry, Prostatic Neoplasms, Cancer, Prostate-Specific Antigen, medicine.disease, Protein Structure, Tertiary, Cold Temperature, medicine.anatomical_structure, Oncology, Prostatic acid phosphatase, Antigens, Surface, Immunology, Leukocytes, Mononuclear, Peptide vaccine, biology.protein, Protein Tyrosine Phosphatases, Antibody, Peptides, business, T-Lymphocytes, Cytotoxic |
| الوصف: | Purpose: The peptide vaccine candidates identified to date have been focused on the HLA-A2 and HLA-A24 alleles. The HLA-A11, HLA-A31, and HLA-A33 alleles share binding motifs and belong to an HLA-A3 supertype family. In this study, we attempted to identify CTL-directed peptide candidates, derived from prostate-related antigens and shared by HLA-A11+, HLA-A31+, and HLA-A33+ prostate cancer patients.Experimental Design: Based on the binding motif to the HLA-A3 supertype alleles, 42 peptides were prepared from prostate-specific antigen (PSA), prostate-specific membrane antigen (PSMA), and prostatic acid phosphatase (PAP). These peptides were first screened for their ability to be recognized by immunoglobulin G (IgG) of prostate cancer patients and subsequently for the potential to induce peptide-specific and prostate cancer–reactive CTLs from peripheral blood mononuclear cells (PBMC) of cancer patients with the HLA-A11, HLA-A31, and HLA-A33 alleles.Results: Five peptide candidates, including the PSA16-24, PAP155-163, PAP248-257, PSMA207-215, and PSMA431-440 peptides, were frequently recognized by IgGs of prostate cancer patients. These peptides efficiently induced peptide-specific and prostate cancer–reactive CTLs from PBMCs of cancer patients with the HLA-A11, HLA-A31, and HLA-A33 alleles. Antibody blocking and cold inhibition experiments revealed that the HLA-A3 supertype–restricted cytotoxicity against prostate cancer cells could be ascribed to peptide-specific and CD8+ T cells.Conclusions: We identified prostate-related antigen-derived new peptide candidates for HLA-A11-, HLA-A31-, and HLA-A33-positive prostate cancer patients. This information could facilitate the development of a peptide-based anticancer vaccine for patients with alleles other than HLA-A2 and HLA-A24. |
| تدمد: | 1557-3265 1078-0432 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0727988e9eadc2e662bbca13c82777b1Test https://doi.org/10.1158/1078-0432.ccr-05-0682Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0727988e9eadc2e662bbca13c82777b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15573265 10780432 |
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