Abstract 1736: Skewed usage of TCRVbeta repertoire and predictive role of CD60 and CD49d expression in the survival rate of patients with Sézàry Syndrome
| العنوان: | Abstract 1736: Skewed usage of TCRVbeta repertoire and predictive role of CD60 and CD49d expression in the survival rate of patients with Sézàry Syndrome |
|---|---|
| المؤلفون: | Adriano Mari, Marina Liso, Deborah Pomponi, Enrico Scala, Elisabetta Caprini, Giuseppe Alfonso Lombardo, Maria Grazia Narducci, Giandomenico Russo |
| المصدر: | Cancer Research. 70:1736-1736 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cancer Research, Oncology, Expression (architecture), Repertoire, Biology, Bioinformatics, CD49d, Survival rate |
| الوصف: | Background: Sézary Syndrome (SS) is a rare and aggressive leukemic variant of Cutaneous T-Cell Lymphoma (CTCL) characterised by erythroderma and the presence of a malignant circulating memory CD4+ T cell clone with skin homing characteristics, lack of CD26 and CD49d and over-expression of CD60. The availability of a large panel of monoclonal antibodies to CDR3 Vß epitopes, allows the study of the TCR repertoire by flow cytometry and a more precise identification of the malignant clone phenotypic characteristics. Objective: The aim of this study was to evaluate both the distribution of TCR-Vß family and CD60/CD49d expression within the clonal T cell population in patients affected by SS. Methods: We studied, by five-color flow cytometry analysis, TCR-Vß repertoire in 62 patients affected by SS, in 180 patients with MF, 6 cases of B cell Lymphomas (BCL), 19 with chronic Eczema (CE) recruited and followed from 1998 to 2008. A sequential gating strategy was used in order to examine the CD60 and CR49d expression within the neoplastic clonal population identified through the TCR-Vß repertoire analysis. Results and Conclusion: A skewed usage of only 4 out of 25 TCR-Vß subfamilies was observed in circulating T cell clone in 61.9% of patients affected by SS. TCR-Vß 2 and 5.1 were the most frequently represented (42.8% of TCR-Vß reactive patients), followed by TCR-Vß 12 and 13.1. In 20 cases the TCR-Vß panel was unable to identify the neoplastic clone, recognizing less than 5% of T cells, thus representing an indirect proof for the presence of a clonal population. CD60 was over expressed and CD49d down regulated within clonal T lymphocytes. This phenotype of the dominant T cell clone in the peripheral blood of patients with SS was significantly associated with a lower survival rate (chi-square 5.467, p Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1736. |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::39e4b8847835dbefe7d7e619a89732a9Test https://doi.org/10.1158/1538-7445.am10-1736Test |
| رقم الانضمام: | edsair.doi...........39e4b8847835dbefe7d7e619a89732a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
|---|